PAR-4 Antibody (5F4): sc-293206

PAR-4 Antibody (5F4) is a mouse monoclonal IgG1 kappa light chain antibody that detects PAR-4 protein of human origin by western blotting (WB), immunoprecipitation (IP), and enzyme-linked immunosorbent assay (ELISA). Anti-PAR-4 antibody (5F4) is available as the non-conjugated format. PAR-4, also known as Proteinase-Activated Receptor 4, plays a crucial role in regulating platelet activation and thrombus formation, making PAR-4 essential for maintaining hemostasis. As a member of the protease-activated receptor family, PAR-4 is activated by thrombin, a serine protease that cleaves the receptor, leading to a cascade of intracellular signaling events that ultimately result in increased intracellular calcium levels and platelet aggregation. This process is vital for the body′s response to vascular injury, as PAR-4 facilitates the formation of a stable blood clot to prevent excessive bleeding. Additionally, PAR-4′s interaction with other proteins, such as PAR-1 and PAR-2, highlights PAR-4′s importance in the complex network of signaling pathways involved in thrombosis and inflammation. Understanding PAR-4 function and interactions is critical for developing therapeutic strategies targeting coagulation disorders and inflammatory diseases.

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PAR-4 Antibody (5F4) References:

1. Protease-activated receptors 1 and 4 mediate activation of human platelets by thrombin.  |  Kahn, ML., et al. 1999. J Clin Invest. 103: 879-87. PMID: 10079109
2. Evidence for the presence of a protease-activated receptor distinct from the thrombin receptor in human keratinocytes.  |  Santulli, RJ., et al. 1995. Proc Natl Acad Sci U S A. 92: 9151-5. PMID: 7568091
3. Agonist recognition by proteinase-activated receptor 2 and thrombin receptor. Importance of extracellular loop interactions for receptor function.  |  Lerner, DJ., et al. 1996. J Biol Chem. 271: 13943-7. PMID: 8662993
4. The proteinase-activated receptor 2 is induced by inflammatory mediators in human endothelial cells. Comparison with the thrombin receptor.  |  Nystedt, S., et al. 1996. J Biol Chem. 271: 14910-5. PMID: 8663011
5. The human proteinase-activated receptor-3 (PAR-3) gene. Identification within a Par gene cluster and characterization in vascular endothelial cells and platelets.  |  Schmidt, VA., et al. 1998. J Biol Chem. 273: 15061-8. PMID: 9614115
6. Cloning and characterization of human protease-activated receptor 4.  |  Xu, WF., et al. 1998. Proc Natl Acad Sci U S A. 95: 6642-6. PMID: 9618465
7. Thrombin.  |  Goldsack, NR., et al. 1998. Int J Biochem Cell Biol. 30: 641-6. PMID: 9695019
8. Analysis of a Ca2+-activated K+ channel that mediates hyperpolarization via the thrombin receptor pathway.  |  Sullivan, R., et al. 1998. Am J Physiol. 275: C1342-8. PMID: 9814983