Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
PAR-3 CRISPR/Cas9 KO Plasmid (m): sc-420266
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- PAR-3 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the PAR-3 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: PAR-3 Antibody (G-4): sc-393127
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PAR-3 CRISPR/Cas9 KO Plasmid (m) | sc-420266 | 20 µg | $397.00 |
Overview
Mouse F2rl2 encodes protease-activated receptor-3 (PAR-3), a member of the GPCR family activated in the context of extracellular protease signaling and coagulation-associated cues. PAR-3 participates in protease-activated receptor networks that can engage heterotrimeric G-protein signaling and coordinate downstream pathways influencing calcium flux, MAPK responses, and cytoskeletal remodeling in responsive cell types. In murine systems, F2rl2 is studied for its roles in vascular and inflammatory biology, platelet-associated signaling, and stromal-immune communication within tissue microenvironments. Dysregulated protease receptor signaling is relevant to models of thrombosis, inflammation, and cancer biology, where altered receptor expression or signaling tone can reshape cell migration and barrier-associated processes.
PAR-3 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the F2rl2 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the F2rl2 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the F2rl2 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish PAR-3 protein expression.
This CRISPR knockout system enables efficient generation of F2rl2-deficient cell models for investigation of PAR-3 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting F2rl2 exon(s) critical for PAR-3 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple F2rl2 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by PAR-3 CRISPR/Cas9 KO Plasmid (m) and PAR-3 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the F2rl2 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by PAR-3 HDR Plasmid (m) and PAR-3 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by F2rl2 homology arms to support homology-directed repair at defined F2rl2 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.