PAR-2 CRISPR/Cas9 KO Plasmid (m2): sc-420265-KO-2

Overview

F2rl1 encodes protease-activated receptor-2 (PAR-2), a G protein–coupled receptor activated by serine protease cleavage that exposes a tethered ligand, initiating signaling through Gq/11, Gi/o, and G12/13 pathways. PAR-2 stimulation mobilizes intracellular Ca2+, engages MAPK/ERK and NF-κB cascades, and promotes transcriptional programs linked to cytokine production, barrier regulation, and nociceptive sensitization. In mouse tissues, PAR-2 is broadly expressed in epithelial and endothelial compartments, immune cells, and sensory neurons, where it coordinates inflammatory and tissue remodeling responses. Dysregulated PAR-2 activity has been implicated in models of airway and intestinal inflammation, dermatitis, pain, and tumor microenvironment signaling, making it a useful target for mechanistic studies of protease-driven biology.

PAR-2 CRISPR/Cas9 KO Plasmid (m2) is a pool of plasmids designed for targeted disruption of the F2rl1 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the F2rl1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.

The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the F2rl1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish PAR-2 protein expression.

This CRISPR knockout system enables efficient generation of F2rl1-deficient cell models for investigation of PAR-2 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.

Key Features

• sgRNAs targeting F2rl1 exon(s) critical for PAR-2 function
• Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
• GFP reporter for identification of transfected cells
• Pool of plasmids targeting multiple F2rl1 genomic sites to improve knockout efficiency
• Compatible with delivery by transfection

Design Variants

CRISPRs +/- HDRs

• gRNAs encoded by PAR-2 CRISPR/Cas9 KO Plasmid (m) and PAR-2 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the F2rl1 locus. One or both targeting designs may be available. See Related Products for availability.
• HDR donor constructs encoded by PAR-2 HDR Plasmid (m) and PAR-2 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by F2rl1 homology arms to support homology-directed repair at defined F2rl1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.