PAPSS 1 Antibody (A-2): sc-376244
- PAPSS 1 Antibody (A-2) is a mouse monoclonal IgG2b κ PAPSS 1 antibody, cited in 1 publications, provided at 200 µg/ml
- raised against amino acids 288-329 mapping within an internal region of PAPSS 1 of human origin
- PAPSS 1 Antibody (A-2) is recommended for detection of PAPSS 1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including equine
- Anti-PAPSS 1 Antibody (A-2) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG2b BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for PAPSS 1 Antibody (A-2) for WB and IHC(P) applications. These reagents are now offered in bundles with PAPSS 1 Antibody (A-2) (see ordering information below).
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PAPSS 1 Antibody (A-2) is a mouse monoclonal IgG2b kappa light chain antibody that detects PAPSS 1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Anti-PAPSS 1 antibody (A-2) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. PAPSS 1 plays a crucial role in the biosynthesis of sulfated L-selectin ligands in endothelial cells, which is essential for cell adhesion and signaling processes in the immune response. PAPSS 1 is a bifunctional enzyme that catalyzes two key steps in the sulfate activation pathway, making PAPSS 1 vital for sulfate assimilation in the sulfur metabolism pathway. PAPSS 1 is primarily expressed in the pancreas, liver, testis, thymus, kidney, prostate, ovary, and small intestine, highlighting PAPSS 1′s importance in various physiological processes. Additionally, PAPSS 1 expression is regulated by chlorate inhibition, which can impact PAPSS 1 activity and, consequently, the sulfation processes PAPSS 1 mediates.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
PAPSS 1 Antibody (A-2) References:
- Human 3'-phosphoadenosine 5'-phosphosulfate (PAPS) synthase: biochemistry, molecular biology and genetic deficiency. | Venkatachalam, KV. 2003. IUBMB Life. 55: 1-11. PMID: 12716056
- Pharmacogenetics of human 3'-phosphoadenosine 5'-phosphosulfate synthetase 1 (PAPSS1): gene resequencing, sequence variation, and functional genomics. | Xu, ZH., et al. 2003. Biochem Pharmacol. 65: 1787-96. PMID: 12781330
- The crystal structure of human PAPS synthetase 1 reveals asymmetry in substrate binding. | Harjes, S., et al. 2005. J Mol Biol. 347: 623-35. PMID: 15755455
- Sulfation in high endothelial venules: cloning and expression of the human PAPS synthetase. | Girard, JP., et al. 1998. FASEB J. 12: 603-12. PMID: 9576487
- cDNA cloning, expression, and characterization of the human bifunctional ATP sulfurylase/adenosine 5'-phosphosulfate kinase enzyme. | Yanagisawa, K., et al. 1998. Biosci Biotechnol Biochem. 62: 1037-40. PMID: 9648242
- Site-selected mutagenesis of a conserved nucleotide binding HXGH motif located in the ATP sulfurylase domain of human bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthase. | Venkatachalam, KV., et al. 1999. J Biol Chem. 274: 2601-4. PMID: 9915785
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PAPSS 1 Antibody (A-2) | sc-376244 | 200 µg/ml | $322.00 | |||
PAPSS 1 Antibody (A-2): m-IgGκ BP-HRP Bundle | sc-522889 | 200 µg Ab, 40 µg BP | $361.00 | |||
PAPSS 1 Antibody (A-2): m-IgG2b BP-HRP Bundle | sc-549079 | 200 µg Ab; 10 µg BP | $361.00 | |||
PAPSS 1 Antibody (A-2) AC | sc-376244 AC | 500 µg/ml, 25% agarose | $424.00 | |||
PAPSS 1 Antibody (A-2) HRP | sc-376244 HRP | 200 µg/ml | $322.00 | |||
PAPSS 1 Antibody (A-2) FITC | sc-376244 FITC | 200 µg/ml | $336.00 | |||
PAPSS 1 Antibody (A-2) PE | sc-376244 PE | 200 µg/ml | $349.00 | |||
PAPSS 1 Antibody (A-2) Alexa Fluor® 488 | sc-376244 AF488 | 200 µg/ml | $364.00 | |||
PAPSS 1 Antibody (A-2) Alexa Fluor® 546 | sc-376244 AF546 | 200 µg/ml | $364.00 | |||
PAPSS 1 Antibody (A-2) Alexa Fluor® 594 | sc-376244 AF594 | 200 µg/ml | $364.00 | |||
PAPSS 1 Antibody (A-2) Alexa Fluor® 647 | sc-376244 AF647 | 200 µg/ml | $364.00 | |||
PAPSS 1 Antibody (A-2) Alexa Fluor® 680 | sc-376244 AF680 | 200 µg/ml | $364.00 | |||
PAPSS 1 Antibody (A-2) Alexa Fluor® 790 | sc-376244 AF790 | 200 µg/ml | $364.00 |