PAPP-A Antibody (B-7): sc-365226

PAPP-A Antibody (B-7) is a mouse monoclonal IgG1 kappa light chain antibody that detects PAPP-A protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). PAPP-A (B-7) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Pregnancy-associated plasma protein-A (Pappalysin-1 or PAPP-A), also known as Insulin-like growth factor-dependent IGF-binding protein 4 (IGFBP4) protease, is a member of the peptidase M43B family of proteins and plays a crucial role in regulating insulin-like growth factor (IGF) availability by cleaving IGFBP4 and IGFBP5, thereby releasing bound IGF. This function is particularly important as IGF is a key regulator of growth and development, influencing cellular proliferation and differentiation. PAPP-A is primarily expressed in the placenta, specifically in the septa and anchoring villi, and PAPP-A levels increase throughout pregnancy, making PAPP-A a valuable biomarker for monitoring pregnancy health. Additionally, lower levels of PAPP-A can be detected in tissues such as the kidney, prostate, breast, ovary, and endometrium, highlighting PAPP-A′s broader biological significance. PAPP-A is a secreted protein that can form homodimers, and in pregnancy serum, PAPP-A may also form a heterotetramer with PRG-2, further underscoring PAPP-A′s complex role in reproductive biology.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

PAPP-A Antibody (B-7) References:

1. Proteolytic degradation of insulin-like growth factor binding proteins by ovarian follicles: a control mechanism for selection of dominant follicles.  |  Spicer, LJ. 2004. Biol Reprod. 70: 1223-30. PMID: 14668213
2. Pregnancy-associated plasma protein-A (PAPP-A) expression and insulin-like growth factor binding protein-4 protease activity in normal and malignant ovarian surface epithelial cells.  |  Kalli, KR., et al. 2004. Int J Cancer. 110: 633-40. PMID: 15146551
3. Follicular development: the role of the follicular microenvironment in selection of the dominant follicle.  |  Fortune, JE., et al. 2004. Anim Reprod Sci. 82-83: 109-26. PMID: 15271447
4. Low pregnancy-associated plasma protein-a at 10(+1) to 14(+6) weeks of gestation and a possible mechanism leading to miscarriage.  |  Santolaya-Forgas, J., et al. 2004. Fetal Diagn Ther. 19: 456-61. PMID: 15305104
5. IGFBP-4 degradation by pregnancy-associated plasma protein-A in MC3T3 osteoblasts.  |  Bunn, RC., et al. 2004. Biochem Biophys Res Commun. 325: 698-706. PMID: 15541345
6. Pregnancy-associated plasma protein-A and insulin-like growth factor binding protein mRNAs in granulosa cells of dominant and subordinate follicles of preovulatory cattle.  |  Santiago, CA., et al. 2005. Domest Anim Endocrinol. 28: 46-63. PMID: 15620806
7. Pregnancy-associated plasma protein-A levels in patients with acute coronary syndromes: comparison with markers of systemic inflammation, platelet activation, and myocardial necrosis.  |  Heeschen, C., et al. 2005. J Am Coll Cardiol. 45: 229-37. PMID: 15653020
8. Identification of two novel human genes, DIPLA1 and DIPAS, expressed in placenta tissue.  |  García, J. and Castrillo, JL. 2005. Gene. 344: 241-50. PMID: 15656990
9. PAPP-A and the IGF system.  |  Monget, P. and Oxvig, C. 2016. Ann Endocrinol (Paris). 77: 90-6. PMID: 27155776
10. PAPP-A: a promising therapeutic target for healthy longevity.  |  Conover, CA. and Oxvig, C. 2017. Aging Cell. 16: 205-209. PMID: 28035757
11. PAPP-A as a Potential Target in Thyroid Eye Disease.  |  Conover, CA., et al. 2024. J Clin Endocrinol Metab. 109: 3119-3125. PMID: 38752390
12. Amino acid sequence of human pregnancy-associated plasma protein-A derived from cloned cDNA.  |  Kristensen, T., et al. 1994. Biochemistry. 33: 1592-8. PMID: 7508748

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-PAPP-A Antibody (B-7) has 6 citations in a variety of scientific publications.

Primary antibodies like Anti-PAPP-A Antibody (B-7) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

PAPP-A Antibody (B-7) Specifications

Alternate Product Name: PAPP-A

Product Clone Name: B-7

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 666-840

Epitope Target: PAPP-A

Epitope Species: human

Product Reactivity/Detection: PAPP-A

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 6 citations in various scientific publications

Available Conjugates/Forms for PAPP-A Antibody (B-7): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin