PAP-2a Antibody (A-4): sc-515517

PAP-2a Antibody (A-4) is a mouse monoclonal IgM antibody that detects PAP-2a of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-PAP-2a antibody (A-4) is available as the non-conjugated form. PAP-2a, also known as phosphatidic acid phosphatase 2, is a crucial member of the integral membrane glycoprotein family that plays a significant role in lipid metabolism and signal transduction. PAP-2a is involved in the dephosphorylation of lipid phosphates, including phosphatidic acid and lysophosphatidic acid, which are important signaling molecules in various cellular processes such as cell growth, migration, and differentiation. PAP-2a functions independently of magnesium ions and shows insensitivity to N-ethylmaleimide inhibition, highlighting unique biochemical properties as a vital player in the phospholipase D signaling pathway. PAP-2a, along with isoforms PAP-2b and PAP-2c, exhibits distinct tissue distribution patterns, with PAP-2a and PAP-2b being ubiquitously expressed, while PAP-2c is predominantly found in the human brain, placenta, and pancreas. Understanding the functional dynamics of PAP-2a and interactions with other lipid phosphates is essential for elucidating roles in cellular signaling and potential implications in various diseases.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

PAP-2a Antibody (A-4) References:

1. Characterization of the pulmonary N-ethylmaleimide-insensitive phosphatidate phosphohydrolase.  |  Nanjundan, M. and Possmayer, F. 2000. Exp Lung Res. 26: 361-81. PMID: 10914334
2. Aging promotes a different phosphatidic acid utilization in cytosolic and microsomal fractions from brain and liver.  |  Pasquaré, SJ., et al. 2001. Exp Gerontol. 36: 1387-401. PMID: 11602212
3. Expression of ectolipid phosphate phosphohydrolases in 3T3F442A preadipocytes and adipocytes. Involvement in the control of lysophosphatidic acid production.  |  Simon, MF., et al. 2002. J Biol Chem. 277: 23131-6. PMID: 11956205
4. Lipid phosphate phosphatases regulate lysophosphatidic acid production and signaling in platelets: studies using chemical inhibitors of lipid phosphate phosphatase activity.  |  Smyth, SS., et al. 2003. J Biol Chem. 278: 43214-23. PMID: 12909631
5. The lipid phosphatase LPP3 regulates extra-embryonic vasculogenesis and axis patterning.  |  Escalante-Alcalde, D., et al. 2003. Development. 130: 4623-37. PMID: 12925589
6. Differential localization of lipid phosphate phosphatases 1 and 3 to cell surface subdomains in polarized MDCK cells.  |  Jia, YJ., et al. 2003. FEBS Lett. 552: 240-6. PMID: 14527693
7. Lysophosphatidic acid and sphingosine 1-phosphate biology: the role of lipid phosphate phosphatases.  |  Pyne, S., et al. 2004. Semin Cell Dev Biol. 15: 491-501. PMID: 15271294
8. Regulation of cell survival by lipid phosphate phosphatases involves the modulation of intracellular phosphatidic acid and sphingosine 1-phosphate pools.  |  Long, J., et al. 2005. Biochem J. 391: 25-32. PMID: 15960610
9. Lipid phosphate phosphatase-2 activity regulates S-phase entry of the cell cycle in Rat2 fibroblasts.  |  Morris, KE., et al. 2006. J Biol Chem. 281: 9297-306. PMID: 16467304
10. Human type 2 phosphatidic acid phosphohydrolases. Substrate specificity of the type 2a, 2b, and 2c enzymes and cell surface activity of the 2a isoform.  |  Roberts, R., et al. 1998. J Biol Chem. 273: 22059-67. PMID: 9705349

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-PAP-2a Antibody (A-4) has 0 citations in a variety of scientific publications.

Primary antibodies like Anti-PAP-2a Antibody (A-4) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

PAP-2a Antibody (A-4) Specifications

Alternate Product Name: PAP-2a

Product Clone Name: A-4

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgM kappa light chain

Amino Acids: 143-161

Epitope Target: PAP-2a

Epitope Species: mouse

Product Reactivity/Detection: PAP-2a

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgM kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 0 citations in various scientific publications

Available Conjugates/Forms for PAP-2a Antibody (A-4): P: Blocking Peptide