pan hnRNP Antibody (C-6): sc-166577

pan hnRNP Antibody (C-6) is a mouse monoclonal IgG1 kappa light chain antibody that detects pan hnRNP protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). pan hnRNP (C-6) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Heterogeneous nuclear ribonucleoproteins (hnRNPs) play a crucial role in regulating pre-mRNA processing and transport by binding to heterogeneous nuclear RNA (hnRNA), which are transcripts produced by RNA polymerase II. hnRNP complexes are essential components of the spliceosome, with hnRNP A1 being one of the most significant pre-mRNA/mRNA binding proteins and most abundant proteins in the nucleus. hnRNP A1 and A2/B1 interaction with splicing factors is vital for pre-mRNA processing regulation, as these proteins can directly inhibit splicing factor association and influence splice site selection. While most hnRNP proteins localize in the nucleus, hnRNP C1 and C2 can shuttle between the nucleus and cytoplasm, contributing to functional versatility. hnRNPs F and H are closely related proteins that preferentially bind to poly(rG) regions on RNA, highlighting hnRNP interaction specificity. Alternative processing of pre-mRNA and post-translational modifications, including phosphorylation and methylation, further demonstrate hnRNP proteins′ complexity and regulatory potential in gene expression and RNA metabolism.

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pan hnRNP Antibody (C-6) References:

1. Nuclear pre-mRNA compartmentalization: trafficking of released transcripts to splicing factor reservoirs.  |  Melcák, I., et al. 2000. Mol Biol Cell. 11: 497-510. PMID: 10679009
2. All human granzymes target hnRNP K that is essential for tumor cell viability.  |  van Domselaar, R., et al. 2012. J Biol Chem. 287: 22854-64. PMID: 22582387
3. Primary structure of human nuclear ribonucleoprotein particle C proteins: conservation of sequence and domain structures in heterogeneous nuclear RNA, mRNA, and pre-rRNA-binding proteins.  |  Swanson, MS., et al. 1987. Mol Cell Biol. 7: 1731-9. PMID: 3110598
4. LCDR regulates the integrity of lysosomal membrane by hnRNP K-stabilized LAPTM5 transcript and promotes cell survival.  |  Yang, X., et al. 2022. Proc Natl Acad Sci U S A. 119: PMID: 35091468
5. Glioblastoma upregulates SUMOylation of hnRNP A2/B1 to eliminate the tumor suppressor miR-204-3p, accelerating angiogenesis under hypoxia.  |  Guo, Q., et al. 2023. Cell Death Dis. 14: 147. PMID: 36810326
6. Single-Cell RNA Sequencing (scRNA-seq) Identifies L1CAM as a Key Mediator between Epithelial Tuft Cell and Innate Lymphoid Cell in the Colon of Hnrnp I Knockout Mice.  |  Xu, GB., et al. 2023. Biomedicines. 11: PMID: 37893107
7. Identification of RNA-binding protein hnRNP C targeting the 3'UTR of the TAP-associated glycoprotein tapasin in melanoma.  |  Wang, Y. and Seliger, B. 2024. Oncoimmunology. 13: 2370928. PMID: 38948930
8. Heterogeneous nuclear ribonucleoproteins H, H', and F are members of a ubiquitously expressed subfamily of related but distinct proteins encoded by genes mapping to different chromosomes.  |  Honoré, B., et al. 1995. J Biol Chem. 270: 28780-9. PMID: 7499401
9. A nuclear localization domain in the hnRNP A1 protein.  |  Siomi, H. and Dreyfuss, G. 1995. J Cell Biol. 129: 551-60. PMID: 7730395
10. The determinants of RNA-binding specificity of the heterogeneous nuclear ribonucleoprotein C proteins.  |  Görlach, M., et al. 1994. J Biol Chem. 269: 23074-8. PMID: 8083209
11. Identification and characterisation of a novel human RNA-binding protein.  |  Badolato, J., et al. 1995. Gene. 166: 323-7. PMID: 8543184
12. Regulated tissue-specific expression of antagonistic pre-mRNA splicing factors.  |  Hanamura, A., et al. 1998. RNA. 4: 430-44. PMID: 9630249

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-pan hnRNP Antibody (C-6) has 11 citations in a variety of scientific publications.

Primary antibodies like Anti-pan hnRNP Antibody (C-6) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

pan hnRNP Antibody (C-6) Specifications

Alternate Product Name: pan hnRNP

Product Clone Name: C-6

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 61-260

Epitope Target: hnRNP D0

Epitope Species: human

Product Reactivity/Detection: hnRNP D0, hnRNP A/B and JKTBP

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 11 citations in various scientific publications

Available Conjugates/Forms for pan hnRNP Antibody (C-6): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred hnRNP A/B antibody is hnRNP A/B Antibody (G-10): sc-376411.