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pan CEA Antibody (D-3): sc-55547

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Datasheets
  • pan CEA Antibody (D-3) is a mouse monoclonal IgG2a κ, cited in 2 publications, provided at 200 µg/ml
  • raised against amino acids 35-334 mapping near the N-terminus of CEA of human origin
  • recommended for detection of pan CEA of human origin by WB, IP, IF, IHC(P) and ELISA
  • m-IgG Fc BP-HRP, m-IgG2a BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for pan CEA Antibody (D-3) for WB and IHC(P) applications. These reagents are now offered in bundles with pan CEA Antibody (D-3) (see ordering information below).
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pan CEA Antibody (D-3) is a mouse monoclonal IgG2a antibody that detects carcinoembryonic antigen (CEA) in human samples through western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). anti-CEA antibody (D-3) plays a crucial role in cancer diagnostics, as CEA is one of the most widely utilized tumor markers for assessing carcinomas in serum immunoassays. CEA protein is primarily located on the cell surface and is involved in cell adhesion processes, which are vital for maintaining tissue architecture and facilitating communication between cells. This localization is particularly important in tumor biology, as alterations in cell adhesion can lead to increased tumor invasiveness and metastasis. Additionally, human CEACAM proteins, including CEA, are encoded by genes clustered on chromosome 19, and expression levels can provide insights into tumor progression and patient prognosis. CEA (D-3) monoclonal antibody enables researchers to effectively study CEA′s role in various cancer types, contributing to a better understanding of tumor biology and potential therapeutic targets.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA

LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences

pan CEA Antibody (D-3) References:

  1. Carcinoembryonic antigen (CEA) levels and tumor histology in colon cancer.  |  Bivins, BA., et al. 1975. J Surg Res. 18: 257-61. PMID: 1094196
  2. Carcinoembryonic antigen (CEA) presentation and specific T cell-priming by human dendritic cells transfected with CEA-mRNA.  |  Eppler, E., et al. 2002. Eur J Cancer. 38: 184-93. PMID: 11750849
  3. Reaction profiles of seven enzyme immunoassay kits for carcinoembryonic antigen (CEA) analyzed with purified preparations of CEA and related normal antigens.  |  Kuroki, M., et al. 1992. Clin Biochem. 25: 29-35. PMID: 1551238
  4. Computed tomography of pulmonary changes in rheumatoid arthritis: carcinoembryonic antigen (CEA) as a marker of airway disease.  |  Koch, MC., et al. 2016. Rheumatol Int. 36: 531-9. PMID: 26886389
  5. Carcinoembryonic antigen (CEA) in gastrointestinal and extragastrointestinal tumors and its relationship to tumor-cell differentiation.  |  Denk, H., et al. 1972. Int J Cancer. 10: 262-72. PMID: 4350187
  6. Carcinoembryonic antigen (CEA) levels in patients with brain tumours.  |  Miyake, E., et al. 1979. Acta Neurochir (Wien). 46: 53-7. PMID: 452968
  7. Carcinoembryonic antigen (CEA) in colonic cancer. Use in preoperative and postoperative diagnosis and prognosis.  |  Dhar, P., et al. 1972. JAMA. 221: 31-5. PMID: 5067755
  8. Immunological comparison of carcinoembryonic antigen (CEA) extracted from tumours of various organs: their use in radioimmunological CEA determinations.  |  Lamerz, R. and Burtin, P. 1983. Br J Cancer. 47: 823-32. PMID: 6407507
  9. Salivary carcinoembryonic antigen (CEA) in cystic fibrosis.  |  MacSween, JM. and Gillespie, CT. 1980. Pediatr Res. 14: 187-9. PMID: 7383740
  10. Effect of cigarette smoke on the mRNA and protein expression of carcinoembryonic antigen (CEA), a possible chemoattractant for neutrophils in human bronchioloalveolar tissues.  |  Ohwada, A., et al. 1995. Thorax. 50: 651-7. PMID: 7638808

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

pan CEA Antibody (D-3)

sc-55547
200 µg/ml
$322.00

pan CEA Antibody (D-3): m-IgG Fc BP-HRP Bundle

sc-537071
200 µg Ab; 10 µg BP
$361.00

pan CEA Antibody (D-3): m-IgGκ BP-HRP Bundle

sc-534257
200 µg Ab; 40 µg BP
$361.00

pan CEA Antibody (D-3): m-IgG2a BP-HRP Bundle

sc-547927
200 µg Ab; 10 µg BP
$361.00

Can pan CEA (D-3): sc-55547 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: AbPolly
Thank you for your question. Yes, pan CEA (D-3): sc-55547 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-25
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Rated 5 out of 5 by from Produced beautiful immunoperoxidase membraneProduced beautiful immunoperoxidase membrane and cytoplasmic staining in formalin fixed, paraffin-embedded human colon tissue. -SCBT QC
Date published: 2015-02-11
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pan CEA Antibody (D-3) is rated 5.0 out of 5 by 2.
  • y_2026, m_5, d_5, h_11
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.43
  • cp_1, bvpage1
  • co_hasreviews, tv_1, tr_1
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