Paip2b Antibody (E-9): sc-514137
- Paip2b Antibody (E-9) is a mouse monoclonal IgG1 κ provided at 200 µg/ml
- raised against amino acids 1-49 mapping at the N-terminus of Paip2b of human origin
- Anti-Paip2b Antibody (E-9) is recommended for detection of Paip2b of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-Paip2b Antibody (E-9) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Paip2b Antibody (E-9) for WB applications. These reagents are now offered in bundles with Paip2b Antibody (E-9) (see ordering information below).
Paip2b Antibody (E-9) is a mouse monoclonal IgG1 kappa light chain antibody that detects Paip2b in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-Paip2b antibody (E-9) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Paip2b, consisting of 123 amino acids, plays a crucial role in regulating polyadenylate-binding protein (PABP) activity, which is essential for mRNA metabolism, including translation initiation and mRNA stability. PABP interacts with the poly(A) tail of mRNA and translation initiation factor eIF4G, facilitating mRNA circularization, which enhances translation efficiency. Paip2b functions as a translational inhibitor by decreasing PABP′s affinity for polyadenylate RNA, thereby disrupting poly(A) ribonucleoprotein structure. This regulatory mechanism is vital for maintaining proper gene expression and cellular function, ensuring translation is finely tuned in response to cellular conditions. Paip2b interactions with other PABP-interacting proteins, such as PaiP1, which acts as a translational stimulator, highlight the complex network of regulation governing mRNA translation and stability.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Paip2b Antibody (E-9) References:
- Multiple portions of poly(A)-binding protein stimulate translation in vivo. | Gray, NK., et al. 2000. EMBO J. 19: 4723-33. PMID: 10970864
- Translational repression by a novel partner of human poly(A) binding protein, Paip2. | Khaleghpour, K., et al. 2001. Mol Cell. 7: 205-16. PMID: 11172725
- Structure and function of the C-terminal PABC domain of human poly(A)-binding protein. | Kozlov, G., et al. 2001. Proc Natl Acad Sci U S A. 98: 4409-13. PMID: 11287632
- Dual interactions of the translational repressor Paip2 with poly(A) binding protein. | Khaleghpour, K., et al. 2001. Mol Cell Biol. 21: 5200-13. PMID: 11438674
- Fructose modulates GLUT5 mRNA stability in differentiated Caco-2 cells: role of cAMP-signalling pathway and PABP (polyadenylated-binding protein)-interacting protein (Paip) 2. | Gouyon, F., et al. 2003. Biochem J. 375: 167-74. PMID: 12820898
- Regulation of poly(A) binding protein function in translation: Characterization of the Paip2 homolog, Paip2B. | Berlanga, JJ., et al. 2006. RNA. 12: 1556-68. PMID: 16804161
- Regulation of poly(A)-binding protein through PABP-interacting proteins. | Derry, MC., et al. 2006. Cold Spring Harb Symp Quant Biol. 71: 537-43. PMID: 17381337
- Crystallization and preliminary X-ray diffraction analysis of the middle domain of Paip1. | Kanaan, AS., et al. 2009. Acta Crystallogr Sect F Struct Biol Cryst Commun. 65: 1060-4. PMID: 19851022
- Interaction of polyadenylate-binding protein with the eIF4G homologue PAIP enhances translation. | Craig, AW., et al. 1998. Nature. 392: 520-3. PMID: 9548260
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Paip2b Antibody (E-9) | sc-514137 | 200 µg/ml | $316.00 | |||
Paip2b Antibody (E-9): m-IgG Fc BP-HRP Bundle | sc-531040 | 200 µg Ab; 10 µg BP | $354.00 | |||
Paip2b Antibody (E-9): m-IgGκ BP-HRP Bundle | sc-524618 | 200 µg Ab, 40 µg BP | $354.00 | |||
Paip2b Antibody (E-9): m-IgG1 BP-HRP Bundle | sc-544370 | 200 µg Ab; 20 µg BP | $354.00 | |||
Paip2b Antibody (E-9) AC | sc-514137 AC | 500 µg/ml, 25% agarose | $416.00 | |||
Paip2b Antibody (E-9) HRP | sc-514137 HRP | 200 µg/ml | $316.00 | |||
Paip2b Antibody (E-9) FITC | sc-514137 FITC | 200 µg/ml | $330.00 | |||
Paip2b Antibody (E-9) PE | sc-514137 PE | 200 µg/ml | $343.00 | |||
Paip2b Antibody (E-9) Alexa Fluor® 488 | sc-514137 AF488 | 200 µg/ml | $357.00 | |||
Paip2b Antibody (E-9) Alexa Fluor® 546 | sc-514137 AF546 | 200 µg/ml | $357.00 | |||
Paip2b Antibody (E-9) Alexa Fluor® 594 | sc-514137 AF594 | 200 µg/ml | $357.00 | |||
Paip2b Antibody (E-9) Alexa Fluor® 647 | sc-514137 AF647 | 200 µg/ml | $357.00 | |||
Paip2b Antibody (E-9) Alexa Fluor® 680 | sc-514137 AF680 | 200 µg/ml | $357.00 | |||
Paip2b Antibody (E-9) Alexa Fluor® 790 | sc-514137 AF790 | 200 µg/ml | $357.00 |