PAFAH1B3 Antibody (D-8): sc-393612
- PAFAH1B3 Antibody (D-8) is a mouse monoclonal IgG2a κ PAFAH1B3 antibody, cited in 2 publications, provided at 200 µg/ml
- raised against amino acids 181-231 mapping at the C-terminus of PAFAH1B3 of human origin
- PAFAH1B3 Antibody (D-8) is recommended for detection of PAFAH1B3 of human origin by WB, IP, IF and ELISA
- Anti-PAFAH1B3 Antibody (D-8) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG2a BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for PAFAH1B3 Antibody (D-8) for WB applications. These reagents are now offered in bundles with PAFAH1B3 Antibody (D-8) (see ordering information below).
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PAFAH1B3 Antibody (D-8) is a mouse monoclonal IgG2a kappa light chain antibody that detects PAFAH1B3 protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-PAFAH1B3 antibody (D-8) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. PAFAH1B3 protein, also known as the 29kDa subunit of the platelet-activating factor acetylhydrolase complex, plays a crucial role in the hydrolysis of the sn-2 ester bond of platelet-activating factor (PAF) and related pro-inflammatory phospholipids, thereby modulating their bioactivity and influencing inflammatory responses. PAFAH1B3 protein is primarily located in the cytoplasm and is essential for maintaining cellular homeostasis, particularly in the brain, where PAFAH1B3 contributes to neuronal development and function. PAFAH1B3 subunit interacts with PAFAH1B2 to form a heterodimer, which is vital for the enzyme′s stability and catalytic activity. Proper functioning of PAFAH1B3 is critical, as dysregulation has been implicated in various neurological disorders and inflammatory conditions.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
PAFAH1B3 Antibody (D-8) References:
- PAF-acetylhydrolases. | Derewenda, ZS. and Ho, YS. 1999. Biochim Biophys Acta. 1441: 229-36. PMID: 10570251
- Lissencephaly associated mutations suggest a requirement for the PAFAH1B heterotrimeric complex in brain development. | Sweeney, KJ., et al. 2000. Mech Dev. 92: 263-71. PMID: 10727864
- Functional hemizygosity of PAFAH1B3 due to a PAFAH1B3-CLK2 fusion gene in a female with mental retardation, ataxia and atrophy of the brain. | Nothwang, HG., et al. 2001. Hum Mol Genet. 10: 797-806. PMID: 11285245
- Previously uncharacterized roles of platelet-activating factor acetylhydrolase 1b complex in mouse spermatogenesis. | Yan, W., et al. 2003. Proc Natl Acad Sci U S A. 100: 7189-94. PMID: 12775763
- The Pafah1b complex interacts with the reelin receptor VLDLR. | Zhang, G., et al. 2007. PLoS One. 2: e252. PMID: 17330141
- Novel isoforms of intracellular platelet activating factor acetylhydrolase (PAFAH1b2) in human testis; encoded by alternatively spliced mRNAs. | Scott, BT., et al. 2008. Prostaglandins Other Lipid Mediat. 85: 69-80. PMID: 18155631
- The structure and function of platelet-activating factor acetylhydrolases. | Derewenda, ZS. and Derewenda, U. 1998. Cell Mol Life Sci. 54: 446-55. PMID: 9645224
- The beta and gamma subunits of the human platelet-activating factor acetyl hydrolase isoform Ib (PAFAH1B2 and PAFAH1B3) map to chromosome 11q23 and 19q13.1, respectively. | Moro, F., et al. 1998. Genomics. 51: 157-9. PMID: 9693049
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PAFAH1B3 Antibody (D-8) | sc-393612 | 200 µg/ml | $322.00 | |||
PAFAH1B3 Antibody (D-8): m-IgG Fc BP-HRP Bundle | sc-530520 | 200 µg Ab; 10 µg BP | $361.00 | |||
PAFAH1B3 Antibody (D-8): m-IgGκ BP-HRP Bundle | sc-524024 | 200 µg Ab, 40 µg BP | $361.00 | |||
PAFAH1B3 Antibody (D-8): m-IgG2a BP-HRP Bundle | sc-547515 | 200 µg Ab; 10 µg BP | $361.00 | |||
PAFAH1B3 Antibody (D-8) AC | sc-393612 AC | 500 µg/ml, 25% agarose | $424.00 | |||
PAFAH1B3 Antibody (D-8) HRP | sc-393612 HRP | 200 µg/ml | $322.00 | |||
PAFAH1B3 Antibody (D-8) FITC | sc-393612 FITC | 200 µg/ml | $336.00 | |||
PAFAH1B3 Antibody (D-8) PE | sc-393612 PE | 200 µg/ml | $349.00 | |||
PAFAH1B3 Antibody (D-8) Alexa Fluor® 488 | sc-393612 AF488 | 200 µg/ml | $364.00 | |||
PAFAH1B3 Antibody (D-8) Alexa Fluor® 546 | sc-393612 AF546 | 200 µg/ml | $364.00 | |||
PAFAH1B3 Antibody (D-8) Alexa Fluor® 594 | sc-393612 AF594 | 200 µg/ml | $364.00 | |||
PAFAH1B3 Antibody (D-8) Alexa Fluor® 647 | sc-393612 AF647 | 200 µg/ml | $364.00 | |||
PAFAH1B3 Antibody (D-8) Alexa Fluor® 680 | sc-393612 AF680 | 200 µg/ml | $364.00 | |||
PAFAH1B3 Antibody (D-8) Alexa Fluor® 790 | sc-393612 AF790 | 200 µg/ml | $364.00 |