Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Paf1 CRISPR/Cas9 KO Plasmid (h): sc-417797
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Paf1 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Paf1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Paf1 Antibody (E-7): sc-514491
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Paf1 CRISPR/Cas9 KO Plasmid (h) | sc-417797 | 20 µg | $397.00 |
Overview
PAF1 encodes Paf1, a core subunit of the RNA polymerase II–associated factor 1 complex (PAF1C) that coordinates transcription elongation with co-transcriptional RNA processing and chromatin regulation. Through interactions with Pol II and elongation machinery, PAF1C influences promoter-proximal pausing, mRNA 3′ end formation, and deposition of histone marks linked to active transcription, including H3K4 and H3K36 methylation. Paf1 contributes to genome stability by modulating transcription–replication conflicts and DNA damage responses, and it participates in control of cell cycle and differentiation programs. Dysregulated PAF1C activity and altered PAF1 expression have been associated with aberrant transcriptional programs observed in multiple cancers and other diseases involving epigenetic and transcriptional misregulation.
Paf1 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the PAF1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the PAF1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the PAF1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Paf1 protein expression.
This CRISPR knockout system enables efficient generation of PAF1-deficient cell models for investigation of Paf1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting PAF1 exon(s) critical for Paf1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple PAF1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Paf1 CRISPR/Cas9 KO Plasmid (h) and Paf1 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the PAF1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Paf1 HDR Plasmid (h) and Paf1 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by PAF1 homology arms to support homology-directed repair at defined PAF1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.