Paf1 Antibody (E-7): sc-514491

Paf1 Antibody (E-7) is a mouse monoclonal IgG1 antibody that detects Paf1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-Paf1 antibody (E-7) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Paf1 plays a crucial role in the regulation of gene expression, particularly in RNA polymerase II-mediated transcription elongation. Paf1 functions as a key component of the Paf1 complex, which is essential for the full expression of a subset of genes in Saccharomyces cerevisiae that respond to signals from the Pkc1/MAP kinase cascade. The Paf1 complex not only facilitates transcription elongation by interacting with other transcription elongation factors, such as Spt16/Pob3 and Spt4/Spt5, but also participates in critical regulatory pathways alongside Swi4/Swi6 and Mbp1/Swi6. Paf1′s interactions and functions underscore its significance in maintaining genomic stability, as deletion of Paf1 or associated proteins can lead to increased recombination events and sensitivity to cell wall-damaging agents. Paf1 (E-7) antibody serves as a valuable tool for researchers investigating the intricate mechanisms of transcription regulation and cellular responses.

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Paf1 Antibody (E-7) References:

1. Ctr9, Rtf1, and Leo1 are components of the Paf1/RNA polymerase II complex.  |  Mueller, CL. and Jaehning, JA. 2002. Mol Cell Biol. 22: 1971-80. PMID: 11884586
2. The Paf1 complex physically and functionally associates with transcription elongation factors in vivo.  |  Squazzo, SL., et al. 2002. EMBO J. 21: 1764-74. PMID: 11927560
3. Phenotypic analysis of Paf1/RNA polymerase II complex mutations reveals connections to cell cycle regulation, protein synthesis, and lipid and nucleic acid metabolism.  |  Betz, JL., et al. 2002. Mol Genet Genomics. 268: 272-85. PMID: 12395202
4. The yeast pafl-rNA polymerase II complex is required for full expression of a subset of cell cycle-regulated genes.  |  Porter, SE., et al. 2002. Eukaryot Cell. 1: 830-42. PMID: 12455700
5. PD2/PAF1 at the Crossroads of the Cancer Network.  |  Karmakar, S., et al. 2018. Cancer Res. 78: 313-319. PMID: 29311159
6. The Paf1 Complex: A Keystone of Nuclear Regulation Operating at the Interface of Transcription and Chromatin.  |  Francette, AM., et al. 2021. J Mol Biol. 433: 166979. PMID: 33811920
7. Role of the Paf1 complex in the maintenance of stem cell pluripotency and development.  |  Park, J., et al. 2023. FEBS J. 290: 951-961. PMID: 35869661
8. PAF1 cooperates with YAP1 in metaplastic ducts to promote pancreatic cancer.  |  Nimmakayala, RK., et al. 2022. Cell Death Dis. 13: 839. PMID: 36180487
9. Elevated PAF1-RAD52 axis confers chemoresistance to human cancers.  |  Rauth, S., et al. 2023. Cell Rep. 42: 112043. PMID: 36709426
10. PAF1/HIF1α axis rewires the glycolytic metabolism to fuel aggressiveness of pancreatic cancer.  |  Ogunleye, AO., et al. 2024. Cancer Metab. 12: 26. PMID: 39242538
11. PAF1-mediated transcriptional reprogramming confers docetaxel resistance in advanced prostate cancer.  |  Muniyan, S., et al. 2025. Cancer Lett. 609: 217355. PMID: 39603380
12. A complex containing RNA polymerase II, Paf1p, Cdc73p, Hpr1p, and Ccr4p plays a role in protein kinase C signaling.  |  Chang, M., et al. 1999. Mol Cell Biol. 19: 1056-67. PMID: 9891041

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Paf1 Antibody (E-7) has 2 citations in a variety of scientific publications.

Primary antibodies like Anti-Paf1 Antibody (E-7) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Paf1 Antibody (E-7) Specifications

Alternate Product Name: Paf1

Product Clone Name: E-7

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁

Amino Acids: 36-59

Epitope Target: Paf1

Epitope Species: human

Product Reactivity/Detection: Paf1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 2 citations in various scientific publications

Available Conjugates/Forms for Paf1 Antibody (E-7): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin, X: TransCruz Reagent