PADI4 Antibody (A-11): sc-365369

PADI4 Antibody (A-11) is a mouse monoclonal IgG2a kappa light chain antibody that detects PADI4 protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). PADI4 (A-11) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. The protein arginine deiminase (PAD) family, which includes PADI4, plays a crucial role in the post-translational modification of proteins by catalyzing the conversion of arginine residues to citrulline in the presence of calcium. This modification is particularly significant in the context of autoimmune diseases, as increased citrullination of proteins has been implicated in the pathogenesis of rheumatoid arthritis. PADI4 is primarily localized in the cytoplasm of eosinophils and neutrophils, where PADI4 contributes to the regulation of immune responses. The gene encoding PADI4 is associated with susceptibility to rheumatoid arthritis, highlighting PADI4′s importance in understanding disease mechanisms and potential therapeutic targets.

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PADI4 Antibody (A-11) References:

1. Localization of peptidylarginine deiminase 4 (PADI4) and citrullinated protein in synovial tissue of rheumatoid arthritis.  |  Chang, X., et al. 2005. Rheumatology (Oxford). 44: 40-50. PMID: 15466895
2. The inhibition of antithrombin by peptidylarginine deiminase 4 may contribute to pathogenesis of rheumatoid arthritis.  |  Chang, X., et al. 2005. Rheumatology (Oxford). 44: 293-8. PMID: 15561738
3. Citrullinated proteins in rheumatoid arthritis.  |  Yamada, R., et al. 2005. Front Biosci. 10: 54-64. PMID: 15574347
4. Comparison of enzymatic properties between hPADI2 and hPADI4.  |  Nakayama-Hamada, M., et al. 2005. Biochem Biophys Res Commun. 327: 192-200. PMID: 15629448
5. Determination of sites citrullinated by peptidylarginine deiminase using 18O stable isotope labeling and mass spectrometry.  |  Kubota, K., et al. 2005. Rapid Commun Mass Spectrom. 19: 683-8. PMID: 15700232
6. Investigation of polymorphisms in the PADI4 gene in determining severity of inflammatory polyarthritis.  |  Barton, A., et al. 2005. Ann Rheum Dis. 64: 1311-5. PMID: 15731287
7. Functional haplotypes of PADI4: relevance for rheumatoid arthritis specific synovial intracellular citrullinated proteins and anticitrullinated protein antibodies.  |  Cantaert, T., et al. 2005. Ann Rheum Dis. 64: 1316-20. PMID: 15760928
8. Genetic and genomic studies of PADI4 in rheumatoid arthritis.  |  Harney, SM., et al. 2005. Rheumatology (Oxford). 44: 869-72. PMID: 15814578
9. Deimination of histone H2A and H4 at arginine 3 in HL-60 granulocytes.  |  Hagiwara, T., et al. 2005. Biochemistry. 44: 5827-34. PMID: 15823041
10. Stimulation of DC-CIK with PADI4 Protein Can Significantly Elevate the Therapeutic Efficiency in Esophageal Cancer.  |  Liu, C., et al. 2019. J Immunol Res. 2019: 6587570. PMID: 30944835
11. A quantitative and site-specific atlas of the citrullinome reveals widespread existence of citrullination and insights into PADI4 substrates.  |  Rebak, AS., et al. 2024. Nat Struct Mol Biol. 31: 977-995. PMID: 38321148
12. Sorafenib-induced macrophage extracellular traps via ARHGDIG/IL4/PADI4 axis confer drug resistance through inhibiting ferroptosis in hepatocellular carcinoma.  |  Huang, X., et al. 2024. Biol Direct. 19: 110. PMID: 39529192

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-PADI4 Antibody (A-11) has 3 citations in a variety of scientific publications.

Primary antibodies like Anti-PADI4 Antibody (A-11) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

PADI4 Antibody (A-11) Specifications

Alternate Product Name: PADI4

Product Clone Name: A-11

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 181-250

Epitope Target: PADI4

Epitope Species: human

Product Reactivity/Detection: PADI4

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 3 citations in various scientific publications

Available Conjugates/Forms for PADI4 Antibody (A-11): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin