PABP Antibody (A-4)recommended for detection of PABP of mouse, rat and human origin, PABPC1L, PABPC3 and PABPC4 of human origin, PABPC2 and PABPC6 of mouse origin and the corresponding rat homologs by WB, IP, IF & ELISA
PABP Antibody (A-4) is a mouse monoclonal IgG3 (kappa light chain) provided at 200 µg/ml
specific for an epitope mapping between amino acids 11-37 near the N-terminus of PABP of human origin
recommended for detection of PABP of mouse, rat and human origin, PABPC1L, PABPC3 and PABPC4 of human origin, PABPC2 and PABPC6 of mouse origin and the corresponding rat homologs by WB, IP, IF and ELISA; also reactive with additional species, including and equine, canine, bovine, porcine and avian
available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; phycoerythrin, FITC, Alexa Fluor® 488 or Alexa Fluor® 647 for IF, IHC(P) and FCM
blocking peptide, sc-166381 P
See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of PABP (A-4): sc-166381.
Every item is shipped based on the best shipping method assessed for the temperature requirements of that specific item. Items are grouped and shipped together whenever
possible, and a separate shipping charge will be included for each shipping method required. Shipping charges listed below are from our US warehouses to the Contiguous US,
Alaska, Hawaii, Canada and Puerto Rico. Shipping charges for countries outside the US and Canada will be determined once order has been received
Please note: We can not ship to PO boxes
Express Blue Ice
Express Dry Ice
Animal Health Prescription Item
SHIPPING METHODS & CHARGES
Ships via FedEx Ground to Contiguous US, Alaska, Canada, Monday through Friday. This method is used for less temperature sensitive items such as lab ware and animal
health products, bulky and/or heavy items
Labware ships FedEx Ground free of charge to the contiguous US
PABP, for Poly(A)-binding protein, is an essential, well-conserved, multifunctional protein involved in translational initiation, mRNA biogenesis and degradation. PABP is required for the shortening of the 3' poly(A) tail of eukaryotic mRNA and translation initiation. The interaction between PABP and eukaryotic translation initiation factor 4G (eIF4G) facilitates translational initiation of polyadenylated mRNAs. This interaction is mediated, at least in part, by elF4G, which bridges the mRNA termini by simultaneously binding PABP and the cap-binding protein, elF4E. With lower affinities, PABP can also associate with non-poly(A) sequences. The physiological consequences of these PABP/RNA interactions are far from clear but may include functions such as translational silencing. PABP is a modular protein, with four N-terminal RNA-binding domains and an extensive C-terminus. During poliovirus infection, cleavage of eIF4GII and PABP have been proposed to contribute to complete host translation shutoff. The human PABP gene maps to chromosome 8q22.2-q23 and encodes a 633 amino acid protein.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
What application is the blocking peptide sc-166381 P appropriate for?
Asked by: jenni
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here:
Rated 5 out of
Immunofluorescence - mouse brainThe antibody worked amazingly well. Free-floating section of 20um, from adult mouse brain, with PFA perfusion and 6h post-fixation. Slices were treated with citrate and X-100, and blocked for 30min in 5%FBS. Primary antibody was diluted 1:100 in 5%FBS in PBS.
Date published: 2018-05-04
Rated 4 out of
Positive signal in WB in mouse transfectedPositive signal in WB in mouse transfected 293T whole cell lysates. -SCBT QC
Date published: 2015-05-09
Rated 5 out of
Exceptional immunofluorescence cytoplasmicExceptional immunofluorescence cytoplasmic staining in methanol-fixed NIH/3T3 cells. -SCBT QC
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