p68 RNA Helicase Antibody (D-7): sc-365164

p68 RNA Helicase Antibody (D-7) is a mouse monoclonal IgG2a kappa light chain antibody that detects p68 RNA Helicase protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). p68 RNA Helicase Antibody (D-7) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. p68 RNA helicase protein, also known as DEAD-Box Helicase 5, plays a crucial role in RNA metabolism, exhibiting RNA-dependent ATPase activity essential for unwinding RNA structures during processes such as transcription and translation. This activity facilitates proper processing of pre-mRNA and regulation of gene expression. p68 RNA helicase participates in organ differentiation during development and is expressed in early neural development, highlighting significance in embryonic growth and tissue formation. p68 RNA helicase localizes to the nucleus under normal conditions, interacting with transcriptional coactivators such as CBP and p300, further emphasizing a role in gene regulation. Phosphorylation by protein kinase C can inhibit p68 RNA helicase activity, indicating post-translational modifications significantly influence function. During late telophase, p68 RNA helicase colocalizes with fibrillarin in nascent nucleoli, suggesting involvement in ribosome biogenesis. p68 RNA Helicase Antibody (D-7) serves as a valuable tool for researchers studying RNA metabolism and gene regulation in various biological contexts.

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p68 RNA Helicase Antibody (D-7) References:

1. Purification and identification of p68 RNA helicase acting as a transcriptional coactivator specific for the activation function 1 of human estrogen receptor alpha.  |  Endoh, H., et al. 1999. Mol Cell Biol. 19: 5363-72. PMID: 10409727
2. The nuclear DEAD box RNA helicase p68 interacts with the nucleolar protein fibrillarin and colocalizes specifically in nascent nucleoli during telophase.  |  Nicol, SM., et al. 2000. Exp Cell Res. 257: 272-80. PMID: 10837141
3. p68, a DEAD-box RNA helicase, is expressed in chordate embryo neural and mesodermal tissues.  |  Seufert, DW., et al. 2000. J Exp Zool. 288: 193-204. PMID: 11069138
4. Synergism between p68 RNA helicase and the transcriptional coactivators CBP and p300.  |  Rossow, KL. and Janknecht, R. 2003. Oncogene. 22: 151-6. PMID: 12527917
5. The highly related DEAD box RNA helicases p68 and p72 exist as heterodimers in cells.  |  Ogilvie, VC., et al. 2003. Nucleic Acids Res. 31: 1470-80. PMID: 12595555
6. P68 RNA helicase mediates PDGF-induced epithelial mesenchymal transition by displacing Axin from beta-catenin.  |  Yang, L., et al. 2006. Cell. 127: 139-55. PMID: 17018282
7. P68 RNA helicase unwinds the human let-7 microRNA precursor duplex and is required for let-7-directed silencing of gene expression.  |  Salzman, DW., et al. 2007. J Biol Chem. 282: 32773-9. PMID: 17724023
8. p68 RNA helicase promotes glioma cell proliferation in vitro and in vivo via direct regulation of NF-κB transcription factor p50.  |  Wang, R., et al. 2012. Neuro Oncol. 14: 1116-24. PMID: 22810421
9. RNA helicase activity associated with the human p68 protein.  |  Hirling, H., et al. 1989. Nature. 339: 562-4. PMID: 2471939
10. Structural characterization of β-catenin and RX-5902 binding to phospho-p68 RNA helicase by molecular dynamics simulation.  |  Ali, W., et al. 2018. Prog Biophys Mol Biol. 140: 79-89. PMID: 29729328
11. Regulation of p68 RNA helicase by calmodulin and protein kinase C.  |  Buelt, MK., et al. 1994. J Biol Chem. 269: 29367-70. PMID: 7525583
12. Expression of the 'dead box' RNA helicase p68 is developmentally and growth regulated and correlates with organ differentiation/maturation in the fetus.  |  Stevenson, RJ., et al. 1998. J Pathol. 184: 351-9. PMID: 9664900

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-p68 RNA Helicase Antibody (D-7) has 10 citations in a variety of scientific publications.

Primary antibodies like Anti-p68 RNA Helicase Antibody (D-7) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

p68 RNA Helicase Antibody (D-7) Specifications

Alternate Product Name: DDX5

Product Clone Name: D-7

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 547-579

Epitope Target: p68 RNA Helicase

Epitope Species: human

Product Reactivity/Detection: p68 RNA Helicase

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: equine, canine and bovine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 10 citations in various scientific publications

Available Conjugates/Forms for p68 RNA Helicase Antibody (D-7): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin