p54/nrb Antibody (3): sc-136296

p54/nrb Antibody (3) is a mouse monoclonal IgG1 antibody that detects p54/nrb in mouse, rat, human, and canine samples through applications such as western blotting (WB), immunoprecipitation (IP), and immunofluorescence (IF). p54/nrb, a crucial nuclear RNA-binding protein, plays a significant role in the formation and function of paraspeckles, which are essential for the regulation of gene expression and RNA processing. These discrete bodies are located in the interchromatin nucleoplasmic space and are involved in the dynamic movement of RNA-binding proteins, including p54/nrb, between the nucleolus and paraspeckles. The presence of p54/nrb in paraspeckles is vital for the proper accumulation of splicing factors, as p54/nrb co-localizes with splicing speckles, thereby influencing pre-mRNA splicing pathways. p54/nrb contains two RNA recognition motifs and shares extensive homology with the human splicing factor PSF and Drosophila NONA/BJ6, which is essential for normal vision and courtship behaviors. The shared DBHS (Drosophila behavior, human splicing) domain among these proteins suggests a conserved mechanism that may regulate various pathways at the level of pre-mRNA splicing, highlighting the importance of p54/nrb in cellular processes and its potential implications in understanding splicing-related diseases.

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p54/nrb Antibody (3) References:

1. p54(nrb) associates with the 5' splice site within large transcription/splicing complexes.  |  Kameoka, S., et al. 2004. EMBO J. 23: 1782-91. PMID: 15057275
2. Transcriptional activity of androgen receptor is modulated by two RNA splicing factors, PSF and p54nrb.  |  Dong, X., et al. 2007. Mol Cell Biol. 27: 4863-75. PMID: 17452459
3. The mitotic phosphorylation of p54(nrb) modulates its RNA binding activity.  |  Bruelle, C., et al. 2011. Biochem Cell Biol. 89: 423-33. PMID: 21819346
4. Decreased expression of P54(nrb) /NonO correlates with collagen deposition and fibrosis in human aortic dissection.  |  Ren, Z., et al. 2014. Histopathology. 65: 570-80. PMID: 24720418
5. New Noncoding Lytic Transcripts Derived from the Epstein-Barr Virus Latency Origin of Replication, oriP, Are Hyperedited, Bind the Paraspeckle Protein, NONO/p54nrb, and Support Viral Lytic Transcription.  |  Cao, S., et al. 2015. J Virol. 89: 7120-32. PMID: 25926645
6. Regulation of PCGEM1 by p54/nrb in prostate cancer.  |  Ho, TT., et al. 2016. Sci Rep. 6: 34529. PMID: 27682980
7. Silencing of Non-POU-domain-containing octamer-binding protein stabilizes atherosclerotic plaque in apolipoprotein E-knockout mice via NF-κB signaling pathway.  |  Zhang, K., et al. 2018. Int J Cardiol. 263: 96-103. PMID: 29673854
8. The caspase-2 substrate p54nrb exhibits a multifaceted role in tumor cell death susceptibility via gene regulatory functions.  |  Eichler, M., et al. 2022. Cell Death Dis. 13: 386. PMID: 35444189
9. IKZF4/NONO-RAB11FIP3 axis promotes immune evasion in gastric cancer via facilitating PD-L1 endosome recycling.  |  Weng, N., et al. 2024. Cancer Lett. 584: 216618. PMID: 38211652
10. The pleiotropic nature of NONO, a master regulator of essential biological pathways in cancers.  |  Ronchetti, D., et al. 2024. Cancer Gene Ther. 31: 984-994. PMID: 38493226

The preferred p54/nrb antibody is p54/nrb Antibody (G-1): sc-376865.