p53 Antibody (DO-7): sc-47698

p53 Antibody (DO-7) is a mouse monoclonal antibody of the IgG2b kappa chain that specifically targets human p53 protein, recognizing an epitope located within amino acids 1-45 of p53 of human origin. p53 monoclonal mouse antibody (DO-7) is both sensitive and specific in detecting wild-type and mutant forms of p53 under denaturing and non-denaturing conditions. Extensively used in research applications, particularly in immunohistochemistry on formalin-fixed, paraffin-embedded (FFPE) tissues, anti-p53 antibody (DO-7) effectively detects p53 overexpression in various human tumors. p53 monoclonal antibody (DO-7) is validated for use in western blotting, immunoprecipitation, immunofluorescence, immunohistochemistry, and flow cytometry. The p53 protein, also known as TP53, is a crucial tumor suppressor protein involved in regulating cell cycle arrest, apoptosis, senescence, DNA repair, and metabolic responses to cellular stress. It functions as a transcription factor, activating genes that contribute to these processes. The activity of p53 is tightly regulated by MDM2, an E3 ubiquitin ligase that ubiquitinates p53, leading to its proteasomal degradation. Mutations in the DNA-binding domain of p53 (amino acids 110-286) are commonly found in human cancers and can impair its tumor suppressor functions. p53 Antibody (DO-7) is available in an unconjugated form as well as multiple conjugated forms, including agarose, HRP, PE, FITC, and various Alexa Fluor® conjugates, providing versatility for multiple applications.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

p53 Antibody (DO-7) References:

1. p53-dependent cell cycle control: response to genotoxic stress.  |  Schwartz, D. and Rotter, V. 1998. Semin Cancer Biol. 8: 325-36. PMID: 10101798
2. Plasma p53 protein and anti-p53 antibody expression in vinyl chloride monomer workers in Taiwan.  |  Luo, JC., et al. 1999. J Occup Environ Med. 41: 521-6. PMID: 10390705
3. p300/CBP/p53 interaction and regulation of the p53 response.  |  Grossman, SR. 2001. Eur J Biochem. 268: 2773-8. PMID: 11358491
4. The p53 pathway: positive and negative feedback loops.  |  Harris, SL. and Levine, AJ. 2005. Oncogene. 24: 2899-908. PMID: 15838523
5. p53: a new player in reproduction.  |  Hu, W., et al. 2008. Cell Cycle. 7: 848-52. PMID: 18414047
6. Cellular adaptation to hypoxia and p53 transcription regulation.  |  Zhao, Y., et al. 2009. J Zhejiang Univ Sci B. 10: 404-10. PMID: 19434769
7. Current strategies to target p53 in cancer.  |  Chen, F., et al. 2010. Biochem Pharmacol. 80: 724-30. PMID: 20450892
8. The tumor suppressor p53: from structures to drug discovery.  |  Joerger, AC. and Fersht, AR. 2010. Cold Spring Harb Perspect Biol. 2: a000919. PMID: 20516128
9. Long Non-coding RNA, PANDA, Contributes to the Stabilization of p53 Tumor Suppressor Protein.  |  Kotake, Y., et al. 2016. Anticancer Res. 36: 1605-11. PMID: 27069137
10. Transcriptional Regulation by Wild-Type and Cancer-Related Mutant Forms of p53.  |  Pfister, NT. and Prives, C. 2017. Cold Spring Harb Perspect Med. 7: PMID: 27836911

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-p53 Antibody (DO-7) has 255 citations in a variety of scientific publications.

Primary antibodies like Anti-p53 Antibody (DO-7) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

p53 Antibody (DO-7) Specifications

Alternate Product Name: TP53

Product Clone Name: DO-7

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 1-45 20-25 epitope mapped

Epitope Target: p53

Epitope Species: human

Product Reactivity/Detection: both wildtype and mutant p53 under denaturing and non-denaturing conditions

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), FCM

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 255 citations in various scientific publications

Available Conjugates/Forms for p53 Antibody (DO-7): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred p53 antibody is p53 Antibody (DO-1): sc-126.