p44S10 Antibody (E-12): sc-393580

p44S10 Antibody (E-12) is a mouse monoclonal IgG1 kappa light chain antibody that detects p44S10 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). anti-p44S10 antibody (E-12) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. p44S10 plays a crucial role in regulating the 26S proteasome, a key component of cellular machinery responsible for selective protein degradation. This process is vital for maintaining cellular homeostasis, regulating cell cycle, and modulating various signaling pathways. p44S10, also known as S5a, is a highly conserved regulatory subunit of the proteasome expressed in multiple tissues, including heart, liver, skeletal muscle, and pancreas. Expression is not limited to normal tissues but also found in several melanoma cell lines, such as MCF-7, 451Lu, and WM164. Notably, forced expression of p44S10 in radial growth phase melanoma cells enhances cellular proliferation, suggesting p44S10 may serve as a critical link between proteasome activity regulation and tumor cell growth in vivo. p44S10 (E-12) antibody serves as an invaluable tool for researchers investigating protein degradation mechanisms and cancer biology implications.

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p44S10 Antibody (E-12) References:

1. The p44S10 locus, encoding a subunit of the proteasome regulatory particle, is amplified during progression of cutaneous malignant melanoma.  |  Ren, S., et al. 2000. Oncogene. 19: 1419-27. PMID: 10723133
2. The 26S proteasome: a molecular machine designed for controlled proteolysis.  |  Voges, D., et al. 1999. Annu Rev Biochem. 68: 1015-68. PMID: 10872471
3. Developmentally regulated, alternative splicing of the Rpn10 gene generates multiple forms of 26S proteasomes.  |  Kawahara, H., et al. 2000. EMBO J. 19: 4144-53. PMID: 10921894
4. Identification of Nipsnap1 as a novel auxiliary protein inhibiting TRPV6 activity.  |  Schoeber, JP., et al. 2008. Pflugers Arch. 457: 91-101. PMID: 18392847
5. Neuronal localization of the mitochondrial protein NIPSNAP1 in rat nervous system.  |  Nautiyal, M., et al. 2010. Eur J Neurosci. 32: 560-9. PMID: 20646061
6. Identification of NIPSNAP1 as a nocistatin-interacting protein involving pain transmission.  |  Okuda-Ashitaka, E., et al. 2012. J Biol Chem. 287: 10403-10413. PMID: 22311985
7. Involvement of NIPSNAP1, a neuropeptide nocistatin-interacting protein, in inflammatory pain.  |  Okamoto, K., et al. 2016. Mol Pain. 12: PMID: 27030720
8. Mitophagy Receptors in Tumor Biology.  |  Xie, Y., et al. 2020. Front Cell Dev Biol. 8: 594203. PMID: 33262988
9. Purification and characterization of the antisecretory factor: a protein in the central nervous system and in the gut which inhibits intestinal hypersecretion induced by cholera toxin.  |  Lönnroth, I. and Lange, S. 1986. Biochim Biophys Acta. 883: 138-44. PMID: 3524692
10. The clarithromycin-binding proteins NIPSNAP1 and 2 regulate cytokine production through mitochondrial quality control.  |  Yamamoto, S., et al. 2024. Sci Rep. 14: 2354. PMID: 38287119
11. Molecular cloning and expression of a pituitary gland protein modulating intestinal fluid secretion.  |  Johansson, E., et al. 1995. J Biol Chem. 270: 20615-20. PMID: 7657640
12. Structure and functions of the 20S and 26S proteasomes.  |  Coux, O., et al. 1996. Annu Rev Biochem. 65: 801-47. PMID: 8811196

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-p44S10 Antibody (E-12) has 0 citations in a variety of scientific publications.

Primary antibodies like Anti-p44S10 Antibody (E-12) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

p44S10 Antibody (E-12) Specifications

Alternate Product Name: p44S10

Product Clone Name: E-12

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 10-194

Epitope Target: p44S10

Epitope Species: human

Product Reactivity/Detection: p44S10

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 0 citations in various scientific publications

Available Conjugates/Forms for p44S10 Antibody (E-12): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin