p38 beta Antibody (BB10) recommended for detection of p38β of mouse, rat and human origin by WB and IP

p38β Antibody (BB10): sc-33689

p38β Antibody (BB10) is rated 4.0 out of 5 by 3.
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Datasheets

    • p38β Antibody (BB10) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against recombinant p38 of human origin
    • recommended for detection of p38β of mouse, rat and human origin by WB and IP; may cross-react with p38α, p38γ and p38δ
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • See p38α/β (A-12): sc-7972 for p38β antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
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What blocking solution should I use with p38β (BB10): sc-33689 monoclonal antibody for Western blot?

Asked by: Trav11
Thank you for your question. We recommend blocking with Blotto (either TBS Blotto A: sc-2333 or TBS Blotto B: sc-2335). Our Western blot protocol can be found here: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-03-01
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Rated 4 out of 5 by from Specificity demonstrated by Western Blot Specificity demonstrated by Western Blot analysis of human p38 transfected 293T cells and those that have not been transfected to over-express this protein. A definite increase in signal was observed. -SCBT QC
Date published: 2015-05-12
Rated 5 out of 5 by from Published data by WB obtained using human Published data by WB obtained using human Jurkat cells. -SCBT Publication Review
Date published: 2015-03-19
Rated 3 out of 5 by from Strong oval shaped band observed by Western Strong oval shaped band observed by Western Blot using human recombinant p38 protein. -SCBT QC
Date published: 2014-08-12
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