p35 Antibody (B-1): sc-518009
- p35 Antibody (B-1) is a mouse monoclonal IgG1 κ, cited in 2 publications, provided at 200 µg/ml
- specific for an epitope mapping between amino acids 282-307 at the C-terminus of p35 of human origin
- Anti-p35 Antibody (B-1) is recommended for detection of p25 and p35 regulatory subunits of Cdk5 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
- Anti-p35 Antibody (B-1) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for p35 Antibody (B-1) for WB and IHC(P) applications. These reagents are now offered in bundles with p35 Antibody (B-1) (see ordering information below).
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SEE ALSO...
p35 Antibody (B-1) is a mouse monoclonal IgG1 kappa light chain antibody that detects p35 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). p35 (B-1) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. p35 protein plays a crucial role as a regulatory subunit of cyclin-dependent kinase-5 (Cdk5), which is essential for neuronal function and development. Cdk5 is unique among cyclin-dependent kinases as Cdk5 is primarily active in post-mitotic neurons, where Cdk5 regulates processes such as neuronal migration, synaptic plasticity, and maintenance of neuronal architecture. The interaction between p35 and Cdk5 is vital for activation of Cdk5′s kinase activity, and p35 presence is critical for proper functioning of Cdk5 in the brain. p35 can be cleaved to form p25, a truncated form that can lead to aberrant Cdk5 activity and has been implicated in neurodegenerative diseases, highlighting p35′s importance in maintaining neuronal health and function.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
p35 Antibody (B-1) References:
- A family of human cdc2-related protein kinases. | Meyerson, M., et al. 1992. EMBO J. 11: 2909-17. PMID: 1639063
- Toxoplasma gondii: expression pattern and detection of infection using full-length recombinant P35 antigen. | Lu, B., et al. 2006. Exp Parasitol. 113: 83-90. PMID: 16458298
- Suppression of calpain-dependent cleavage of the CDK5 activator p35 to p25 by site-specific phosphorylation. | Kamei, H., et al. 2007. J Biol Chem. 282: 1687-94. PMID: 17121855
- Quantitative measurement of in vivo phosphorylation states of Cdk5 activator p35 by Phos-tag SDS-PAGE. | Hosokawa, T., et al. 2010. Mol Cell Proteomics. 9: 1133-43. PMID: 20097924
- Universal control mechanism regulating onset of M-phase. | Nurse, P. 1990. Nature. 344: 503-8. PMID: 2138713
- Cell cycle control in eukaryotes: molecular mechanisms of cdc2 activation. | Draetta, G. 1990. Trends Biochem Sci. 15: 378-83. PMID: 2147521
- p34cdc2: the S and M kinase? | Pines, J. and Hunter, T. 1990. New Biol. 2: 389-401. PMID: 2149647
- Dominoes and clocks: the union of two views of the cell cycle. | Murray, AW. and Kirschner, MW. 1989. Science. 246: 614-21. PMID: 2683077
- Vps35 Deficiency Impairs Cdk5/p35 Degradation and Promotes the Hyperphosphorylation of Tau Protein in Retinal Ganglion Cells. | Gao, L., et al. 2020. Invest Ophthalmol Vis Sci. 61: 1. PMID: 31995153
- p35 is a neural-specific regulatory subunit of cyclin-dependent kinase 5. | Tsai, LH., et al. 1994. Nature. 371: 419-23. PMID: 8090221
- A brain-specific activator of cyclin-dependent kinase 5. | Lew, J., et al. 1994. Nature. 371: 423-6. PMID: 8090222
- The cdk5/p35 kinase is essential for neurite outgrowth during neuronal differentiation. | Nikolic, M., et al. 1996. Genes Dev. 10: 816-25. PMID: 8846918
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
p35 Antibody (B-1) | sc-518009 | 200 µg/ml | $322.00 | |||
p35 Antibody (B-1): m-IgG Fc BP-HRP Bundle | sc-528077 | 200 µg Ab; 10 µg BP | $361.00 | |||
p35 Antibody (B-1): m-IgG1 BP-HRP Bundle | sc-533450 | 200 µg Ab; 20 µg BP | $361.00 | |||
p35 Antibody (B-1) AC | sc-518009 AC | 500 µg/ml, 25% agarose | $424.00 | |||
p35 Antibody (B-1) HRP | sc-518009 HRP | 200 µg/ml | $322.00 | |||
p35 Antibody (B-1) FITC | sc-518009 FITC | 200 µg/ml | $336.00 | |||
p35 Antibody (B-1) PE | sc-518009 PE | 200 µg/ml | $349.00 | |||
p35 Antibody (B-1) Alexa Fluor® 488 | sc-518009 AF488 | 200 µg/ml | $364.00 | |||
p35 Antibody (B-1) Alexa Fluor® 546 | sc-518009 AF546 | 200 µg/ml | $364.00 | |||
p35 Antibody (B-1) Alexa Fluor® 594 | sc-518009 AF594 | 200 µg/ml | $364.00 | |||
p35 Antibody (B-1) Alexa Fluor® 647 | sc-518009 AF647 | 200 µg/ml | $364.00 | |||
p35 Antibody (B-1) Alexa Fluor® 680 | sc-518009 AF680 | 200 µg/ml | $364.00 | |||
p35 Antibody (B-1) Alexa Fluor® 790 | sc-518009 AF790 | 200 µg/ml | $364.00 |