P2X7 Antibody (D-1): sc-514962
- P2X7 Antibody (D-1) is a mouse monoclonal IgG2a κ P2X7 antibody, cited in 27 publications, provided at 200 µg/ml
- specific for an epitope mapping between amino acids 81-106 within an internal region of P2X7 of human origin
- P2X7 Antibody (D-1) is recommended for detection of P2X7 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-P2X7 Antibody (D-1) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG2a BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for P2X7 Antibody (D-1) for WB applications. These reagents are now offered in bundles with P2X7 Antibody (D-1) (see ordering information below).
QUICK LINKS
P2X7 Antibody (D-1) is a mouse monoclonal IgG2a kappa light chain antibody that detects P2X7 of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). P2X7 (D-1) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. P2X7 is a member of the P2X receptor family, which consists of ligand-gated ion channels that facilitate calcium ion influx in response to extracellular ATP. P2X7 is predominantly located in the plasma membrane of various cell types, including neurons and immune cells, where P2X7 plays a crucial role in mediating synaptic transmission and inflammatory responses. P2X7 can trigger pro-inflammatory cytokine release and is implicated in processes such as necrosis and apoptosis when exposed to high ATP concentrations. Understanding P2X7 function and location is vital for elucidating P2X7′s role in various physiological and pathological conditions, including neurodegenerative diseases and chronic inflammation.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
P2X7 Antibody (D-1) References:
- Cytolytic P2X purinoceptors. | Di Virgilio, F., et al. 1998. Cell Death Differ. 5: 191-9. PMID: 10200464
- Allosteric modulation and accelerated resensitization of human P2X(3) receptors by cibacron blue. | Alexander, K., et al. 1999. J Pharmacol Exp Ther. 291: 1135-42. PMID: 10565834
- Inactivation of P2X2 purinoceptors by divalent cations. | Ding, S. and Sachs, F. 2000. J Physiol. 522 Pt 2: 199-214. PMID: 10639098
- Evidence for P2X(3), P2X(4), P2X(5) but not for P2X(7) containing purinergic receptors in Müller cells of the rat retina. | Jabs, R., et al. 2000. Brain Res Mol Brain Res. 76: 205-10. PMID: 10762695
- A natural dominant negative P2X1 receptor due to deletion of a single amino acid residue. | Oury, C., et al. 2000. J Biol Chem. 275: 22611-4. PMID: 10816552
- P2X receptors in sensory neurones. | Burnstock, G. 2000. Br J Anaesth. 84: 476-88. PMID: 10823099
- Pharmacology of cloned P2X receptors. | North, RA. and Surprenant, A. 2000. Annu Rev Pharmacol Toxicol. 40: 563-80. PMID: 10836147
- Function of the P2X7 receptor in hematopoiesis and leukemogenesis. | He, X., et al. 2021. Exp Hematol. 104: 40-47. PMID: 34687808
- Involvement of P2X7 receptors in chronic pain disorders. | Ren, WJ. and Illes, P. 2022. Purinergic Signal. 18: 83-92. PMID: 34799827
- The P2X7 receptor in mucosal adaptive immunity. | Grassi, F. and Marino, R. 2024. Purinergic Signal. 20: 9-19. PMID: 37067746
- Untangling Macropore Formation and Current Facilitation in P2X7. | Cevoli, F., et al. 2023. Int J Mol Sci. 24: PMID: 37446075
- The human P2x1 receptor: molecular cloning, tissue distribution, and localization to chromosome 17. | Longhurst, PA., et al. 1996. Biochim Biophys Acta. 1308: 185-8. PMID: 8809107
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
P2X7 Antibody (D-1) | sc-514962 | 200 µg/ml | $322.00 | |||
P2X7 Antibody (D-1): m-IgGκ BP-HRP Bundle | sc-524886 | 200 µg Ab, 40 µg BP | $361.00 | |||
P2X7 Antibody (D-1): m-IgG2a BP-HRP Bundle | sc-546176 | 200 µg Ab; 10 µg BP | $361.00 | |||
P2X7 Antibody (D-1) AC | sc-514962 AC | 500 µg/ml, 25% agarose | $424.00 | |||
P2X7 Antibody (D-1) HRP | sc-514962 HRP | 200 µg/ml | $322.00 | |||
P2X7 Antibody (D-1) FITC | sc-514962 FITC | 200 µg/ml | $336.00 | |||
P2X7 Antibody (D-1) PE | sc-514962 PE | 200 µg/ml | $349.00 | |||
P2X7 Antibody (D-1) Alexa Fluor® 488 | sc-514962 AF488 | 200 µg/ml | $364.00 | |||
P2X7 Antibody (D-1) Alexa Fluor® 546 | sc-514962 AF546 | 200 µg/ml | $364.00 | |||
P2X7 Antibody (D-1) Alexa Fluor® 594 | sc-514962 AF594 | 200 µg/ml | $364.00 | |||
P2X7 Antibody (D-1) Alexa Fluor® 647 | sc-514962 AF647 | 200 µg/ml | $364.00 | |||
P2X7 Antibody (D-1) Alexa Fluor® 680 | sc-514962 AF680 | 200 µg/ml | $364.00 | |||
P2X7 Antibody (D-1) Alexa Fluor® 790 | sc-514962 AF790 | 200 µg/ml | $364.00 | |||
P2X7 (D-1) Neutralizing Peptide | sc-514962 P | 100 µg/0.5 ml | $69.00 |