p22-phox Antibody (E-8): sc-271262

p22-phox Antibody (E-8) is a mouse monoclonal IgG1 kappa light chain antibody designed to recognize p22-phox protein, also known as CYBA or cytochrome b-245 alpha chain, from human origin. p22-phox monoclonal antibody (E-8) targets the full-length p22-phox protein, encompassing amino acids 1-195, enabling detection in mouse, rat, and human samples through western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). p22-phox is an integral component of the NADPH oxidase complex, which is essential for the generation of superoxide radicals in phagocytes, neuroepithelial cells, vascular smooth muscle cells, and endothelial cells. Structurally, p22-phox forms a membrane-bound flavocytochrome by pairing with gp91-phox (NOX2), creating the cytochrome b558 complex. This structure facilitates electron transfer necessary for the production of reactive oxygen species, which play a vital role in immune defense and cellular signaling. Additionally, membrane localization of p22-phox allows effective interaction with cytosolic proteins p47-phox and p67-phox during NADPH oxidase activation, leading to assembly of the active enzyme complex. Structural integrity of p22-phox is also important for function as a surface oxygen sensor, initiating signaling pathways in response to hypoxic conditions. Anti-p22-phox antibody (E-8) is available in both non-conjugated form and various conjugated variants, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates, providing flexible options for a wide range of experimental applications. p22-phox (E-8) antibody is an essential tool for researchers studying oxidative stress, immune responses, and related cellular mechanisms, enabling in-depth analysis of the structural and functional dynamics of the NADPH oxidase complex.

The preferred p22-phox antibody is p22-phox Antibody (E-11): sc-271968.