Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
p19 INK4D/CDKN2D CRISPR/Cas9 KO Plasmid (h): sc-401761
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- p19 INK4D/CDKN2D CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the p19 INK4D/CDKN2D genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: p19 INK4D/CDKN2D Antibody (DCS-100): sc-56334
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
p19 INK4D/CDKN2D CRISPR/Cas9 KO Plasmid (h) | sc-401761 | 20 µg | $397.00 |
Overview
CDKN2D encodes p19 INK4D, a member of the INK4 family of cyclin-dependent kinase inhibitors that selectively restrains CDK4/6 activity to maintain RB1 in a hypophosphorylated state and limit G1/S cell-cycle progression. By modulating cyclin D–CDK signaling, p19 INK4D contributes to checkpoint control, proliferation timing, and cellular differentiation programs in multiple tissue contexts. Perturbation of this pathway is frequently linked to dysregulated cell-cycle entry and genome maintenance defects that are relevant to cancer biology and other proliferative disorders. CDKN2D is therefore widely used as a node for dissecting CDK4/6–RB axis regulation, senescence-like states, and stress-responsive growth control.
p19 INK4D/CDKN2D CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the CDKN2D gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the CDKN2D together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the CDKN2D open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish p19 INK4D/CDKN2D protein expression.
This CRISPR knockout system enables efficient generation of CDKN2D-deficient cell models for investigation of p19 INK4D/CDKN2D signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting CDKN2D exon(s) critical for p19 INK4D/CDKN2D function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple CDKN2D genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by p19 INK4D/CDKN2D CRISPR/Cas9 KO Plasmid (h) and p19 INK4D/CDKN2D CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the CDKN2D locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by p19 INK4D/CDKN2D HDR Plasmid (h) and p19 INK4D/CDKN2D HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by CDKN2D homology arms to support homology-directed repair at defined CDKN2D target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.