p130 Cas Antibody (35B.1A4): sc-20029

p130 Cas Antibody (35B.1A4) is a mouse monoclonal IgG1 kappa light chain antibody that detects p130 Cas protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry. p130 Cas (35B.1A4) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. p130 Cas protein, also known as BCAR1 or Crk-associated substrate, plays a crucial role in cellular signaling pathways, particularly in cancer biology. p130 Cas is primarily located in the cytoplasm but can translocate to the cell membrane upon tyrosine phosphorylation, which is essential for function as a signaling scaffold. This translocation allows p130 Cas to interact with various signaling molecules, thereby facilitating signal transmission from the cell surface to the interior, influencing processes such as cell migration, proliferation, and survival. p130 Cas is characterized by a high level of tyrosine phosphorylation and contains multiple SH2-binding motifs, making p130 Cas a potent substrate for protein tyrosine kinases. Interaction with v-Crk and v-Src highlights p130 Cas importance in oncogenic signaling, as these interactions can lead to the activation of downstream pathways that promote tumorigenesis. Using anti-p130 Cas antibody (35B.1A4) in research can provide valuable insights into cell signaling mechanisms and p130 Cas role in various biological processes and diseases.

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p130 Cas Antibody (35B.1A4) References:

1. Analysis of gene expression profile in p130(Cas)-deficient fibroblasts.  |  Nakamoto, T., et al. 2002. Biochem Biophys Res Commun. 294: 635-41. PMID: 12056816
2. Anchorage-independent phosphorylation of p130(Cas) protects lung adenocarcinoma cells from anoikis.  |  Wei, L., et al. 2002. J Cell Biochem. 87: 439-49. PMID: 12397603
3. Tyrosine-phosphorylated epidermal growth factor receptor and cellular p130 provide high affinity binding substrates to analyze Crk-phosphotyrosine-dependent interactions in vitro.  |  Birge, RB., et al. 1992. J Biol Chem. 267: 10588-95. PMID: 1375224
4. Two species of human CRK cDNA encode proteins with distinct biological activities.  |  Matsuda, M., et al. 1992. Mol Cell Biol. 12: 3482-9. PMID: 1630456
5. Binding of transforming protein, P47gag-crk, to a broad range of phosphotyrosine-containing proteins.  |  Matsuda, M., et al. 1990. Science. 248: 1537-9. PMID: 1694307
6. Identification of domains of the v-crk oncogene product sufficient for association with phosphotyrosine-containing proteins.  |  Matsuda, M., et al. 1991. Mol Cell Biol. 11: 1607-13. PMID: 1705010
7. The SH2 and SH3 domains of pp60src direct stable association with tyrosine phosphorylated proteins p130 and p110.  |  Kanner, SB., et al. 1991. EMBO J. 10: 1689-98. PMID: 1710979
8. p130 Crk-associated substrate (CAS) in vascular smooth muscle.  |  Tang, DD. 2009. J Cardiovasc Pharmacol Ther. 14: 89-98. PMID: 19329671
9. Monoclonal antibodies to individual tyrosine-phosphorylated protein substrates of oncogene-encoded tyrosine kinases.  |  Kanner, SB., et al. 1990. Proc Natl Acad Sci U S A. 87: 3328-32. PMID: 2110361
10. CrkI and p130(Cas) complex regulates the migration and invasion of prostate cancer cells.  |  Dai, Y., et al. 2011. Cell Biochem Funct. 29: 625-9. PMID: 22144090
11. Regulation of p130(Cas)/BCAR1 expression in tamoxifen-sensitive and tamoxifen-resistant breast cancer cells by EGR1 and NAB2.  |  Kumbrink, J. and Kirsch, KH. 2012. Neoplasia. 14: 108-20. PMID: 22431919
12. A novel signaling molecule, p130, forms stable complexes in vivo with v-Crk and v-Src in a tyrosine phosphorylation-dependent manner.  |  Sakai, R., et al. 1994. EMBO J. 13: 3748-56. PMID: 8070403

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-p130 Cas Antibody (35B.1A4) has 18 citations in a variety of scientific publications.

Primary antibodies like Anti-p130 Cas Antibody (35B.1A4) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

p130 Cas Antibody (35B.1A4) Specifications

Alternate Product Name: BCAR1

Product Clone Name: 35B.1A4

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids:

Epitope Target: p130 Cas

Epitope Species: human

Product Reactivity/Detection: p130 Cas

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P)

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 18 citations in various scientific publications

Available Conjugates/Forms for p130 Cas Antibody (35B.1A4): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin