p115 Antibody (H-11): sc-48363

p115 Antibody (H-11) is a mouse monoclonal IgG2a kappa light chain antibody that detects p115 of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). anti-p115 antibody (H-11) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. p115, also known as transcytosis-associated protein (TAP), tethering factor, or vesicle docking protein (VDP), plays a crucial role in membrane trafficking, which is essential for maintaining cellular homeostasis and facilitating communication between organelles. Located primarily in the cytosol and associated with the membranes of the Golgi complex, p115 undergoes phosphorylation at Ser942 during interphase, leading to its release from membranes, a process that is vital for the regulation of vesicle fusion and transport. The interactions of p115 with other proteins, such as giantin and GM130, are critical for independent membrane tethering events, highlighting its importance in the secretory and endocytic pathways. Additionally, p115 is recruited to coat protein complex II (COPII) vesicles by Rab1 during their budding from the endoplasmic reticulum, where p115 interacts with a select set of SNAREs, further underscoring its role as a general factor in vesicle transport. The ability of p115 to bind transport vesicles prior to membrane fusion is essential for the proper functioning of cellular transport mechanisms, making p115 a significant target for research in cellular biology and related fields.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

p115 Antibody (H-11) References:

1. Binding relationships of membrane tethering components. The giantin N terminus and the GM130 N terminus compete for binding to the p115 C terminus.  |  Linstedt, AD., et al. 2000. J Biol Chem. 275: 10196-201. PMID: 10744704

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-p115 Antibody (H-11) has 5 citations in a variety of scientific publications.

Primary antibodies like Anti-p115 Antibody (H-11) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

p115 Antibody (H-11) Specifications

Alternate Product Name: p115

Product Clone Name: H-11

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 1-300

Epitope Target: p115

Epitope Species: human

Product Reactivity/Detection: p115

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 5 citations in various scientific publications

Available Conjugates/Forms for p115 Antibody (H-11): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin