p-Stat5a/b Antibody (5G4): sc-81524

p-Stat5a/b Antibody (5G4) is a mouse monoclonal IgG1 antibody that detects p-Stat5a/b, specifically targeting Tyr 694 phosphorylated Stat5a and Tyr 699 phosphorylated Stat5b in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), and immunofluorescence (IF). p-Stat5a/b (5G4) antibody is available in a non-conjugated form, allowing for versatile experimental use. Stat5, or Signal Transducers and Activators of Transcription 5, plays a crucial role in mediating T cell functions, particularly in response to Interleukin-2 (IL-2), which is vital for immune regulation. Upon IL-2 stimulation, both Stat5a and Stat5b undergo rapid phosphorylation at specific tyrosine and serine residues, with Stat5b exhibiting a more prolonged phosphorylation response. This differential phosphorylation is essential for the distinct biological functions of Stat5a and Stat5b, influencing T cell proliferation and survival. Notably, Stat5a is phosphorylated on Tyr694 in a prolactin-sensitive manner, while serine phosphorylation remains constitutive, highlighting the complex regulatory mechanisms governing Stat5 activity. The ability of Stat5 to integrate signals from various cytokines underscores its importance in the immune response, making anti-p-Stat5a/b antibody (5G4) a valuable tool for researchers studying T cell biology and cytokine signaling pathways.

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p-Stat5a/b Antibody (5G4) References:

1. Cytosolic tyrosine dephosphorylation of STAT5. Potential role of SHP-2 in STAT5 regulation.  |  Yu, CL., et al. 2000. J Biol Chem. 275: 599-604. PMID: 10617656
2. Stat5a serine phosphorylation. Serine 779 is constitutively phosphorylated in the mammary gland, and serine 725 phosphorylation influences prolactin-stimulated in vitro DNA binding activity.  |  Beuvink, I., et al. 2000. J Biol Chem. 275: 10247-55. PMID: 10744710
3. Functional uncoupling of the Janus kinase 3-Stat5 pathway in malignant growth of human T cell leukemia virus type 1-transformed human T cells.  |  Kirken, RA., et al. 2000. J Immunol. 165: 5097-104. PMID: 11046040
4. STAT5b, a Mediator of Synergism between c-Src and the Epidermal Growth Factor Receptor.  |  Kloth, MT., et al. 2003. J Biol Chem. 278: 1671-9. PMID: 12429742
5. ERBB4/HER4 potentiates STAT5A transcriptional activity by regulating novel STAT5A serine phosphorylation events.  |  Clark, DE., et al. 2005. J Biol Chem. 280: 24175-80. PMID: 15863494
6. Interleukin 2 signaling involves the phosphorylation of Stat proteins.  |  Frank, DA., et al. 1995. Proc Natl Acad Sci U S A. 92: 7779-83. PMID: 7544001
7. Identification and purification of human Stat proteins activated in response to interleukin-2.  |  Hou, J., et al. 1995. Immunity. 2: 321-9. PMID: 7719937
8. Two discrete regions of interleukin-2 (IL2) receptor beta independently mediate IL2 activation of a PD98059/rapamycin/wortmannin-insensitive Stat5a/b serine kinase.  |  Kirken, RA., et al. 1997. J Biol Chem. 272: 15459-65. PMID: 9182578
9. Differential control of the phosphorylation state of proline-juxtaposed serine residues Ser725 of Stat5a and Ser730 of Stat5b in prolactin-sensitive cells.  |  Yamashita, H., et al. 1998. J Biol Chem. 273: 30218-24. PMID: 9804779
10. Cross-talk between janus kinase-signal transducer and activator of transcription (JAK-STAT) and peroxisome proliferator-activated receptor-alpha (PPARalpha) signaling pathways. Growth hormone inhibition of pparalpha transcriptional activity mediated by stat5b.  |  Zhou, YC. and Waxman, DJ. 1999. J Biol Chem. 274: 2672-81. PMID: 9915797

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-p-Stat5a/b Antibody (5G4) has 12 citations in a variety of scientific publications.

Primary antibodies like Anti-p-Stat5a/b Antibody (5G4) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

p-Stat5a/b Antibody (5G4) Specifications

Alternate Product Name: Stat5a/b

Product Clone Name: 5G4

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁

Amino Acids: Tyr 694

Epitope Target: Tyr 694/699

Epitope Species: human

Product Reactivity/Detection: Tyr 694 phosphorylated Stat5a and Tyr 699 phosphorylated Stat5b

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF

Vialing Information: Each vial contains 50 µg IgG₁ in 0.5 ml of PBS with < 0.1% sodium azide, 0.1% gelatin, PEG and sucrose

Research Citations: 12 citations in various scientific publications

Available Conjugates/Forms for p-Stat5a/b Antibody (5G4): N/A

The preferred Stat5b antibody is Stat5b Antibody (G-2): sc-1656.