p-Stat3 Antibody (B-7) recommended for detection of Tyr 705 phosphorylated Stat3 of mouse, rat and human origin by WB, IP, IF, IHC(P), FCM and ELISA

p-Stat3 Antibody (B-7): sc-8059

p-Stat3 Antibody (B-7) is rated 4.6 out of 5 by 63.
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Datasheets

    • p-Stat3 Antibody (B-7) is a mouse monoclonal IgG2b (kappa light chain) provided at 200 µg/ml
    • Anti-p-Stat3 Antibody (B-7) is recommended for detection of Tyr 705 phosphorylated Stat3 of mouse, rat and human origin by WB, IP, IF, IHC(P), FCM and ELISA
    • Anti-p-Stat3 Antibody (B-7) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to either PerCP or PerCP-Cy5.5 for IF, IHC(P) and FCM
    • TransCruz reagent for Gel Supershift and ChIP applications (sc-8059 X, 200 µg/0.1 ml)
    • blocking peptide, sc-8059 P
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of p-Stat3 (B-7): sc-8059.

What secondary reagent should I use for detection of p-Stat3 Antibody (B-7) via Western blot?

Asked by: Trav11
We recommend using one of our exclusive Mouse IgG Binding Proteins as a secondary detection reagent, such as m-IgGκ BP-HRP: sc-516102, for Western blot. A complete list of all available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Tech Service 8
Date published: 2016-12-15

How should I use the p-Stat3 Antibody (B-7) for immunohistochemical staining if I am working in mouse tissue?

Asked by: Trav11
We recommend using our directly conjugated mouse monoclonal primary antibodies for such purposes. Currently, p-Stat3 Antibody (B-7) is available conjugated to HRP (sc-8059 HRP), phycoerythrin (sc-8059 PE), fluorescein (sc-8059 FITC), Alexa Fluor® 488 (sc-8059 AF488), Alexa Fluor® 647 (sc-8059 AF647), or PerCP (sc-8059 PerCP, 100 tests in 2 ml).
Answered by: Tech Service 8
Date published: 2016-12-15
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Rated 4 out of 5 by from Buena señal. Bandas detectadas en peso molecular. MUestras de celulas estrelladas.
Date published: 2020-05-09
Rated 4 out of 5 by from Good antibody I could detect the band but I felt the molecular weight is slightly different. Well, it was almost good because it could be detected neatly. I diluted 1:1000.
Date published: 2020-03-19
Rated 3 out of 5 by from Fairly good そこそこ使える抗体 Works at 1:200 dilution with HIKARI solution O/N. Clear bands but also some background signals appered in an ovarian cancer cell line. Not recommended for re-probing. NacalaiのHIKARIに1:200で希釈し、4℃で一晩インキュベートして使用。 卵巣癌細胞株で目的のバンドが検出できました。 強度はやや弱く、他にもバンドが出ました。 リプローブしての使用では、同じ条件でも全くバンドが出ませんでした。
Date published: 2020-03-04
Rated 5 out of 5 by from 功能很出色 The p-stat3 Antibody (B-7): sc-8059 provides good data for a multitude of applications including WB, IP, IF, and IHC(P). This antibody has also been cited several times for further verification . We used this antibody for WB, and could see specific bands at 86-91kD.
Date published: 2018-05-25
Rated 5 out of 5 by from Works great! Works great! used at 1:200 for WB using in vivo mouse samples
Date published: 2018-03-22
Rated 5 out of 5 by from Good production It works well We tested the product on the rat brain. The result presented a good band.
Date published: 2018-03-01
Rated 5 out of 5 by from p-stat3 antibody I received a sample of this antibody a month ago and this works as suggested. I am very happy with this purchase and when needed, I will order from SCB again.
Date published: 2018-02-16
Rated 5 out of 5 by from awesome This antibody is awesome, we detected p-Stat3 and Stat3 in HepG2 cell line, and both of them work well!
Date published: 2018-02-14
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