p-Stat1 Antibody (A-2): sc-8394

p-Stat1 Antibody (A-2) is a mouse monoclonal IgG1 kappa light chain antibody that detects Stat1 phosphorylated at Tyr 701 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), flow cytometry (FCM), and enzyme-linked immunosorbent assay (ELISA). p-Stat1 (A-2) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Stat1 plays a crucial role in mediating cellular responses to interferons and other cytokines, functioning as a transcription factor that regulates the expression of genes involved in immune response, cell growth, and apoptosis. The phosphorylation of Stat1 at Tyr 701 is essential for activation and subsequent dimerization with Stat2, forming the ISGF3 complex, which translocates to the nucleus to initiate transcription of interferon-stimulated genes. This process is vital for antiviral response and regulation of immune functions, highlighting the importance of Stat1 in maintaining cellular homeostasis and responding to external stimuli. anti-Stat1 antibody (A-2) accurately detects phosphorylated Stat1, making p-Stat1 (A-2) monoclonal antibody an invaluable tool for researchers studying signaling pathways related to immune responses and cellular regulation.

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p-Stat1 Antibody (A-2) References:

1. STAT1 gain-of-function and chronic demodicosis.  |  Molho-Pessach, V., et al. 2020. Pediatr Dermatol. 37: 153-155. PMID: 31637766
2. The Dual Role of STAT1 in Ovarian Cancer: Insight Into Molecular Mechanisms and Application Potentials.  |  Li, X., et al. 2021. Front Cell Dev Biol. 9: 636595. PMID: 33834023
3. STAT1 N-terminal domain discriminatively controls type I and type II IFN signaling.  |  Göder, A., et al. 2021. Cytokine. 144: 155552. PMID: 34000478
4. STAT1 is required to establish but not maintain interferon-γ-induced transcriptional memory.  |  Tehrani, SS., et al. 2023. EMBO J. 42: e112259. PMID: 37272165
5. Tyrosine-phosphorylated Stat1 and Stat2 plus a 48-kDa protein all contact DNA in forming interferon-stimulated-gene factor 3.  |  Qureshi, SA., et al. 1995. Proc Natl Acad Sci U S A. 92: 3829-33. PMID: 7537377
6. Cooperative transcriptional activity of Jun and Stat3 beta, a short form of Stat3.  |  Schaefer, TS., et al. 1995. Proc Natl Acad Sci U S A. 92: 9097-101. PMID: 7568080
7. Transcriptional responses to polypeptide ligands: the JAK-STAT pathway.  |  Schindler, C. and Darnell, JE. 1995. Annu Rev Biochem. 64: 621-51. PMID: 7574495
8. Interleukin-3, erythropoietin, and prolactin activate a STAT5-like factor in lymphoid cells.  |  Pallard, C., et al. 1995. J Biol Chem. 270: 15942-5. PMID: 7608147
9. Stat4, a novel gamma interferon activation site-binding protein expressed in early myeloid differentiation.  |  Yamamoto, K., et al. 1994. Mol Cell Biol. 14: 4342-9. PMID: 8007943
10. An interleukin-4-induced transcription factor: IL-4 Stat.  |  Hou, J., et al. 1994. Science. 265: 1701-6. PMID: 8085155
11. Stat3: a STAT family member activated by tyrosine phosphorylation in response to epidermal growth factor and interleukin-6.  |  Zhong, Z., et al. 1994. Science. 264: 95-8. PMID: 8140422
12. Jak-STAT pathways and transcriptional activation in response to IFNs and other extracellular signaling proteins.  |  Darnell, JE., et al. 1994. Science. 264: 1415-21. PMID: 8197455

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-p-Stat1 Antibody (A-2) has 107 citations in a variety of scientific publications.

Primary antibodies like Anti-p-Stat1 Antibody (A-2) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

p-Stat1 Antibody (A-2) Specifications

Alternate Product Name: STAT1

Product Clone Name: A-2

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: Tyr 701

Epitope Target: Stat1

Epitope Species: human

Product Reactivity/Detection: Stat1 phosphorylated at Tyr 701

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, FCM, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 107 citations in various scientific publications

Available Conjugates/Forms for p-Stat1 Antibody (A-2): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin, X: TransCruz Reagent

The preferred Stat1 antibody is Stat1 Antibody (C-136): sc-464.