p-SMC1 Antibody (4H374): sc-71790

p-SMC1 Antibody (4H374) is a mouse monoclonal IgG1 antibody that detects p-SMC1 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), and immunofluorescence (IF). p-SMC1, a phosphorylated form of the structural maintenance of chromosomes protein 1, plays a crucial role in the regulation of sister chromatid cohesion and chromosome condensation during mitosis, which is essential for accurate cell division and genomic stability. The phosphorylation of SMC1 is particularly important as it is involved in the DNA damage response, facilitating the repair of double-strand breaks and ensuring that cells do not progress through the cell cycle with damaged DNA. This post-translational modification enhances SMC1′s interaction with other key regulators of the cell cycle, thereby influencing the overall dynamics of chromosomal behavior during mitosis. p-SMC1 is expressed in various human tissues, including the thymus, testis, and colon, highlighting its fundamental role in cellular processes across different biological contexts. anti-p-SMC1 antibody (4H374) effectively recognizes this phosphorylated protein, making p-SMC1 (4H374) monoclonal antibody a valuable tool for researchers studying cell cycle regulation and the mechanisms underlying genomic integrity.

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p-SMC1 Antibody (4H374) References:

1. Involvement of the cohesin protein, Smc1, in Atm-dependent and independent responses to DNA damage.  |  Kim, ST., et al. 2002. Genes Dev. 16: 560-70. PMID: 11877376
2. SMC1 is a downstream effector in the ATM/NBS1 branch of the human S-phase checkpoint.  |  Yazdi, PT., et al. 2002. Genes Dev. 16: 571-82. PMID: 11877377
3. Rapid flow cytometry-based structural maintenance of chromosomes 1 (SMC1) phosphorylation assay for identification of ataxia-telangiectasia homozygotes and heterozygotes.  |  Nahas, SA., et al. 2009. Clin Chem. 55: 463-72. PMID: 19147735
4. PTIP regulates 53BP1 and SMC1 at the DNA damage sites.  |  Wu, J., et al. 2009. J Biol Chem. 284: 18078-84. PMID: 19414588
5. Cohesin phosphorylation and mobility of SMC1 at ionizing radiation-induced DNA double-strand breaks in human cells.  |  Bauerschmidt, C., et al. 2011. Exp Cell Res. 317: 330-7. PMID: 21056556
6. Blood-based detection of radiation exposure in humans based on novel phospho-Smc1 ELISA.  |  Ivey, RG., et al. 2011. Radiat Res. 175: 266-81. PMID: 21388270
7. Phosphorylation of SMC1 by ATR is required for desferrioxamine (DFO)-induced apoptosis.  |  So, EY., et al. 2011. Cell Death Dis. 2: e128. PMID: 21390062
8. Point mutation at the Nbs1 Threonine 278 site does not affect mouse development, but compromises the Chk2 and Smc1 phosphorylation after DNA damage.  |  Li, T. and Wang, ZQ. 2011. Mech Ageing Dev. 132: 382-8. PMID: 21664921
9. Human papillomaviruses activate and recruit SMC1 cohesin proteins for the differentiation-dependent life cycle through association with CTCF insulators.  |  Mehta, K., et al. 2015. PLoS Pathog. 11: e1004763. PMID: 25875106
10. Radiation-Induced Phosphorylation of Serine 360 of SMC1 in Human Peripheral Blood Mononuclear Cells.  |  Park, S., et al. 2019. Radiat Res. 191: 262-270. PMID: 30702968

The preferred SMC1 antibody is SMC1 Antibody (C2M): sc-56776.