p-Shc Antibody (6E10): sc-81520
- p-Shc Antibody (6E10) is a mouse monoclonal IgG1, cited in 5 publications, provided at 50 µg/0.5 ml
- raised against a synthetic phosphopeptide corresponding to amino acids Ser 36 of Shc of human origin
- recommended for detection of Ser 36 phosphorylated Shc of mouse, rat, human and canine origin by WB
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for p-Shc Antibody (6E10). This work is in progress.
QUICK LINKS
SEE ALSO...
p-Shc Antibody (6E10) is a mouse monoclonal IgG1 antibody that detects p-Shc in mouse, rat, human, and canine samples through western blotting (WB). p-Shc, a critical adaptor protein, plays a vital role in cellular signaling pathways by acting as a substrate for receptor tyrosine kinases, which are essential for mediating responses to growth factors. The phosphorylation of specific tyrosine residues on p-Shc, particularly Tyr 239 and Tyr 317, is crucial for its function, as p-Shc facilitates the recruitment of the Grb2-Sos complex, leading to the activation of the Ras/MAPK signaling cascade. This pathway is significant for various cellular processes, including proliferation, differentiation, and survival, highlighting p-Shc′s importance in regulating cellular responses to external stimuli. The human Shc gene is located on chromosome 1q21.3, and Shc expression is tightly regulated, with implications for understanding diseases such as cancer, where aberrant signaling can lead to uncontrolled cell growth. anti-p-Shc antibody (6E10) is an invaluable tool for researchers investigating the dynamics of signaling pathways and p-Shc′s role in various biological contexts.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
p-Shc Antibody (6E10) References:
- Identification of shc as the primary protein binding to the tyrosine-phosphorylated beta 3 subunit of alpha IIbbeta 3 during outside-in integrin platelet signaling. | Cowan, KJ., et al. 2000. J Biol Chem. 275: 36423-9. PMID: 10964917
- Signal transduction through tyrosine-phosphorylated C-terminal fragments of amyloid precursor protein via an enhanced interaction with Shc/Grb2 adaptor proteins in reactive astrocytes of Alzheimer's disease brain. | Russo, C., et al. 2002. J Biol Chem. 277: 35282-8. PMID: 12084708
- p66 Shc and tyrosine-phosphorylated Shc in primary breast tumors identify patients likely to relapse despite tamoxifen therapy. | Frackelton, AR., et al. 2006. Breast Cancer Res. 8: R73. PMID: 17196107
- Molecular dynamics simulations reveal that Tyr-317 phosphorylation reduces Shc binding affinity for phosphotyrosyl residues of epidermal growth factor receptor. | Suenaga, A., et al. 2009. Biophys J. 96: 2278-88. PMID: 19289054
- Phospho-Tyrosine(s) vs. Phosphatidylinositol Binding in Shc Mediated Integrin Signaling. | Lin, X. and Vinogradova, O. 2015. Am J Mol Biol. 5: 17-31. PMID: 25893141
- Exendin-4 inhibits high glucose-induced oxidative stress in retinal pigment epithelial cells by modulating the expression and activation of p66Shc. | Al Sabaani, N. 2021. Cutan Ocul Toxicol. 40: 175-186. PMID: 34275397
- A family of Shc related proteins with conserved PTB, CH1 and SH2 regions. | Pelicci, G., et al. 1996. Oncogene. 13: 633-41. PMID: 8760305
- Distinct mechanisms mediate SHC association with the activated and resting B cell antigen receptor. | D'Ambrosio, D., et al. 1996. Eur J Immunol. 26: 1960-5. PMID: 8765045
- Activation-induced bi-dentate interaction of SHIP and Shc in B lymphocytes. | Pradhan, M. and Coggeshall, KM. 1997. J Cell Biochem. 67: 32-42. PMID: 9328837
- Tyrosine 474 of ZAP-70 is required for association with the Shc adaptor and for T-cell antigen receptor-dependent gene activation. | Pacini, S., et al. 1998. J Biol Chem. 273: 20487-93. PMID: 9685404
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
p-Shc Antibody (6E10) | sc-81520 | 50 µg/0.5 ml | $322.00 |