p-Shc Antibody (1E3): sc-81519

p-Shc Antibody (1E3) is a mouse monoclonal IgG1 antibody that detects p-Shc in mouse, rat, human, and canine samples through applications such as western blotting (WB) and immunoprecipitation (IP). p-Shc, a phosphorylated form of the Shc protein, plays a crucial role in cellular signaling pathways, particularly in growth factor receptor activation. The phosphorylation of specific tyrosine residues, notably Tyr 239 and Tyr 317, is essential for recruiting the Grb2-Sos complex, which subsequently activates the Ras/MAPK signaling cascade. This pathway drives various cellular processes, including cell proliferation, differentiation, and survival, making p-Shc a key player in mediating the effects of growth factors like insulin and epidermal growth factor (EGF). p-Shc′s interaction with other signaling molecules underscores p-Shc′s importance in regulating cellular responses to external stimuli. The human Shc gene is located on chromosome 1q21.3, highlighting evolutionary conservation and significance across species. anti-p-Shc antibody (1E3) serves as an invaluable tool for researchers investigating signal transduction mechanisms and Shc′s role in various physiological and pathological contexts.

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p-Shc Antibody (1E3) References:

1. Identification of shc as the primary protein binding to the tyrosine-phosphorylated beta 3 subunit of alpha IIbbeta 3 during outside-in integrin platelet signaling.  |  Cowan, KJ., et al. 2000. J Biol Chem. 275: 36423-9. PMID: 10964917
2. Signal transduction through tyrosine-phosphorylated carboxy-terminal fragments of APP via an enhanced interaction with Shc/Grb2 adaptor proteins in reactive astrocytes of Alzheimer's disease brain.  |  Russo, C., et al. 2002. Ann N Y Acad Sci. 973: 323-33. PMID: 12485888
3. Role of SHPS-1 in the regulation of insulin-like growth factor I-stimulated Shc and mitogen-activated protein kinase activation in vascular smooth muscle cells.  |  Ling, Y., et al. 2005. Mol Biol Cell. 16: 3353-64. PMID: 15888547
4. p66 Shc and tyrosine-phosphorylated Shc in primary breast tumors identify patients likely to relapse despite tamoxifen therapy.  |  Frackelton, AR., et al. 2006. Breast Cancer Res. 8: R73. PMID: 17196107
5. Molecular dynamics simulations reveal that Tyr-317 phosphorylation reduces Shc binding affinity for phosphotyrosyl residues of epidermal growth factor receptor.  |  Suenaga, A., et al. 2009. Biophys J. 96: 2278-88. PMID: 19289054
6. Adaptor protein Shc acts as an immune-regulator for the LPS-stimulated maturation of bone marrow-derived dendritic cells.  |  Chen, KD., et al. 2011. BMC Immunol. 12: 32. PMID: 21612602
7. Exendin-4 inhibits high glucose-induced oxidative stress in retinal pigment epithelial cells by modulating the expression and activation of p66Shc.  |  Al Sabaani, N. 2021. Cutan Ocul Toxicol. 40: 175-186. PMID: 34275397
8. Specific motifs recognized by the SH2 domains of Csk, 3BP2, fps/fes, GRB-2, HCP, SHC, Syk, and Vav.  |  Songyang, Z., et al. 1994. Mol Cell Biol. 14: 2777-85. PMID: 7511210
9. Activation-induced bi-dentate interaction of SHIP and Shc in B lymphocytes.  |  Pradhan, M. and Coggeshall, KM. 1997. J Cell Biochem. 67: 32-42. PMID: 9328837
10. Gads is a novel SH2 and SH3 domain-containing adaptor protein that binds to tyrosine-phosphorylated Shc.  |  Liu, SK. and McGlade, CJ. 1998. Oncogene. 17: 3073-82. PMID: 9872323

The preferred Shc antibody is Shc Antibody (PG-797): sc-967.