p-RGS9-1 Antibody (A4): sc-18842
- p-RGS9-1 Antibody (A4) is a mouse monoclonal IgG1 κ provided at 200 µg/ml
- raised against a short amino acid sequence containing Ser 475 phosphorylated RGS9-1 of mouse origin
- p-RGS9-1 Antibody (A4) is recommended for detection of Ser 475 phosphorylated RGS9-1 of mouse origin by WB; non cross-reactive with RGS9-2
- Anti-p-RGS9-1 Antibody (A4) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for p-RGS9-1 Antibody (A4) for WB applications. These reagents are now offered in bundles with p-RGS9-1 Antibody (A4) (see ordering information below).
p-RGS9-1 Antibody (A4) is a mouse monoclonal IgG1 kappa light chain antibody that detects Ser 475 phosphorylated RGS9-1 of mouse origin by western blotting (WB). anti-p-RGS9-1 antibody (A4) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. RGS9-1, a member of the regulator of G protein signaling (RGS) family, plays a crucial role in modulating G protein signaling pathways, particularly in the central nervous system. RGS9-1 is primarily located in the cytosol, where RGS9-1 forms complexes with Gβ5 subunits, which are essential for the regulation of G protein-coupled receptor signaling. The interaction between RGS9-1 and Gβ5 is vital for the formation of active GTPase-activating protein complexes that specifically target the Gao subunit, enhancing the hydrolysis of GTP to GDP. This process helps terminate signaling cascades initiated by G protein-coupled receptors, maintaining cellular homeostasis and preventing overstimulation of signaling pathways. p-RGS9-1 (A4) monoclonal antibody′s ability to accurately detect phosphorylated RGS9-1 makes p-RGS9-1 (A4) antibody an invaluable tool for researchers studying G protein signaling dynamics and their implications in various physiological and pathological processes.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
p-RGS9-1 Antibody (A4) References:
- Fidelity of G protein beta-subunit association by the G protein gamma-subunit-like domains of RGS6, RGS7, and RGS11. | Snow, BE., et al. 1999. Proc Natl Acad Sci U S A. 96: 6489-94. PMID: 10339615
- A G-protein signaling network mediated by an RGS protein. | Guan, KL. and Han, M. 1999. Genes Dev. 13: 1763-7. PMID: 10421628
- Emerging roles for RGS proteins in cell signalling. | Hepler, JR. 1999. Trends Pharmacol Sci. 20: 376-82. PMID: 10462761
- Regulators of G protein signaling 6 and 7. Purification of complexes with gbeta5 and assessment of their effects on g protein-mediated signaling pathways. | Posner, BA., et al. 1999. J Biol Chem. 274: 31087-93. PMID: 10521509
- Structural elements of G alpha subunits that interact with G beta gamma, receptors, and effectors. | Conklin, BR. and Bourne, HR. 1993. Cell. 73: 631-41. PMID: 8388779
- RGS9: a regulator of G-protein signalling with specific expression in rat and mouse striatum. | Thomas, EA., et al. 1998. J Neurosci Res. 52: 118-24. PMID: 9556034
- A G protein gamma subunit-like domain shared between RGS11 and other RGS proteins specifies binding to Gbeta5 subunits. | Snow, BE., et al. 1998. Proc Natl Acad Sci U S A. 95: 13307-12. PMID: 9789084
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
p-RGS9-1 Antibody (A4) | sc-18842 | 200 µg/ml | $322.00 | |||
p-RGS9-1 Antibody (A4): m-IgG Fc BP-HRP Bundle | sc-528337 | 200 µg Ab; 10 µg BP | $361.00 | |||
p-RGS9-1 Antibody (A4): m-IgGκ BP-HRP Bundle | sc-520689 | 200 µg Ab, 40 µg BP | $361.00 | |||
p-RGS9-1 Antibody (A4): m-IgG1 BP-HRP Bundle | sc-542875 | 200 µg Ab; 20 µg BP | $361.00 | |||
p-RGS9-1 Antibody (A4) AC | sc-18842 AC | 500 µg/ml, 25% agarose | $424.00 | |||
p-RGS9-1 Antibody (A4) HRP | sc-18842 HRP | 200 µg/ml | $322.00 | |||
p-RGS9-1 Antibody (A4) FITC | sc-18842 FITC | 200 µg/ml | $336.00 | |||
p-RGS9-1 Antibody (A4) PE | sc-18842 PE | 200 µg/ml | $349.00 | |||
p-RGS9-1 Antibody (A4) Alexa Fluor® 488 | sc-18842 AF488 | 200 µg/ml | $364.00 | |||
p-RGS9-1 Antibody (A4) Alexa Fluor® 546 | sc-18842 AF546 | 200 µg/ml | $364.00 | |||
p-RGS9-1 Antibody (A4) Alexa Fluor® 594 | sc-18842 AF594 | 200 µg/ml | $364.00 | |||
p-RGS9-1 Antibody (A4) Alexa Fluor® 647 | sc-18842 AF647 | 200 µg/ml | $364.00 | |||
p-RGS9-1 Antibody (A4) Alexa Fluor® 680 | sc-18842 AF680 | 200 µg/ml | $364.00 | |||
p-RGS9-1 Antibody (A4) Alexa Fluor® 790 | sc-18842 AF790 | 200 µg/ml | $364.00 |