p-Rb Antibody (7F10): sc-56175
- p-Rb Antibody (7F10) is a rat monoclonal IgG2a, cited in 2 publications, provided at 50 µg/0.5 ml
- raised against a short amino acid sequence containing phosphorylated Tyr 356 Rb
- recommended for detection of Tyr 356 phosphorylated Rb of mouse, rat and human origin by WB, IP and IF; non cross-reactive with non-phosphorylated Rb or Rb phosphorylated at other sites
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for p-Rb Antibody (7F10). This work is in progress.
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SEE ALSO...
p-Rb Antibody (7F10) is a rat monoclonal IgG2a antibody that detects retinoblastoma protein (Rb) in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), and immunofluorescence (IF). p-Rb (7F10) antibody specifically recognizes the phosphorylated form of Rb, which plays a crucial role in regulating the cell cycle, differentiation, and apoptosis. Rb protein is primarily located in the nucleus, where Rb functions as a tumor suppressor by controlling the transition from the G1 phase to the S phase of the cell cycle. This regulation is vital for preventing uncontrolled cell proliferation, which can lead to tumorigenesis. Phosphorylation of Rb at specific serine and threonine residues, including Ser 795, is essential for Rb inactivation, allowing the cell cycle to progress. In a hypophosphorylated state, Rb binds to transcription factors and represses the expression of genes that promote cell cycle progression, thereby acting as a critical checkpoint in cellular growth. Anti-Rb antibody (7F10) detection of this post-translational modification makes p-Rb (7F10) monoclonal antibody an invaluable tool for studying cancer development mechanisms and Rb′s role in cell cycle regulation.
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p-Rb Antibody (7F10) References:
- Phosphorylated retinoblastoma protein complexes with pp32 and inhibits pp32-mediated apoptosis. | Adegbola, O. and Pasternack, GR. 2005. J Biol Chem. 280: 15497-502. PMID: 15716273
- Hyper-phosphorylated retinoblastoma protein suppresses telomere elongation. | Takemura, M., et al. 2008. Biosci Biotechnol Biochem. 72: 630-5. PMID: 18256459
- The cell cycle regulator phosphorylated retinoblastoma protein is associated with tau pathology in several tauopathies. | Stone, JG., et al. 2011. J Neuropathol Exp Neurol. 70: 578-87. PMID: 21666500
- Discrete phosphorylated retinoblastoma protein isoform expression in mouse tooth development. | Zhang, W., et al. 2012. J Mol Histol. 43: 281-8. PMID: 22476877
- Phosphorylated retinoblastoma protein (p-Rb) is involved in neuronal apoptosis after traumatic brain injury in adult rats. | Liu, W., et al. 2013. J Mol Histol. 44: 147-58. PMID: 23371354
- Expression of the oncoprotein gankyrin and phosphorylated retinoblastoma protein in human testis and testicular germ cell tumor. | Ando, S., et al. 2014. Int J Urol. 21: 992-8. PMID: 24861729
- Phosphorylated retinoblastoma protein is a potential predictive marker of irinotecan efficacy for colorectal cancer. | Ikai, A., et al. 2016. Int J Oncol. 48: 1297-304. PMID: 26783196
- Phosphorylated RB Promotes Cancer Immunity by Inhibiting NF-κB Activation and PD-L1 Expression. | Jin, X., et al. 2019. Mol Cell. 73: 22-35.e6. PMID: 30527665
- SETDB1 Modulates Degradation of Phosphorylated RB and Anticancer Efficacy of CDK4/6 Inhibitors. | Huang, Z., et al. 2023. Cancer Res. 83: 875-889. PMID: 36637424
- Phosphorylated retinoblastoma protein stimulates DNA polymerase alpha. | Takemura, M., et al. 1997. Oncogene. 15: 2483-92. PMID: 9395244
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
p-Rb Antibody (7F10) | sc-56175 | 50 µg/0.5 ml | $339.00 |