p-PYK2 Antibody (13.Tyr 402): sc-293142

p-PYK2 Antibody (13.Tyr 402) is a mouse monoclonal IgG2b kappa light chain antibody that detects phosphorylated PYK2, specifically at Tyr 402, in human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). anti-p-PYK2 antibody (13.Tyr 402) is available in both conjugated and non-conjugated forms, providing flexibility for various experimental needs. PYK2, or proline-rich tyrosine kinase 2, plays a crucial role in cellular signaling pathways, particularly in the central nervous system where PYK2 is highly expressed. PYK2 is essential for mediating responses to extracellular signals, as PYK2 is rapidly phosphorylated on tyrosine residues, including Tyr 402, in response to stimuli such as heregulin. Phosphorylation at Tyr 402 facilitates the formation of a multiprotein complex that enhances the phosphorylation of p190 RhoGAP by Src, thereby influencing cytoskeletal dynamics and cellular motility. PYK2 contains other important phosphorylation sites, including Tyr 579, Tyr 580, and Tyr 881, which are involved in activation and interaction with signaling molecules, underscoring PYK2′s role as a pivotal regulator in various cellular processes.

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p-PYK2 Antibody (13.Tyr 402) References:

1. RAFTK/Pyk2 tyrosine kinase mediates the association of p190 RhoGAP with RasGAP and is involved in breast cancer cell invasion.  |  Zrihan-Licht, S., et al. 2000. Oncogene. 19: 1318-28. PMID: 10713673
2. Inhibition of the catalytic activity of cell adhesion kinase beta by protein-tyrosine phosphatase-PEST-mediated dephosphorylation.  |  Lyons, PD., et al. 2001. J Biol Chem. 276: 24422-31. PMID: 11337490
3. Different modes and qualities of tyrosine phosphorylation of Fak and Pyk2 during epithelial-mesenchymal transdifferentiation and cell migration: analysis of specific phosphorylation events using site-directed antibodies.  |  Nakamura, K., et al. 2001. Oncogene. 20: 2626-35. PMID: 11420674
4. Antidepressant effect of the calcium-activated tyrosine kinase Pyk2 in the lateral septum.  |  Sheehan, TP., et al. 2003. Biol Psychiatry. 54: 540-51. PMID: 12946883
5. Regulation of focal adhesion-associated protein tyrosine kinase by both cellular adhesion and oncogenic transformation.  |  Guan, JL. and Shalloway, D. 1992. Nature. 358: 690-2. PMID: 1379699
6. Integrin-dependent phosphorylation and activation of the protein tyrosine kinase pp125FAK in platelets.  |  Lipfert, L., et al. 1992. J Cell Biol. 119: 905-12. PMID: 1385445
7. Focal adhesion protein-tyrosine kinase phosphorylated in response to cell attachment to fibronectin.  |  Hanks, SK., et al. 1992. Proc Natl Acad Sci U S A. 89: 8487-91. PMID: 1528852
8. pp125FAK a structurally distinctive protein-tyrosine kinase associated with focal adhesions.  |  Schaller, MD., et al. 1992. Proc Natl Acad Sci U S A. 89: 5192-6. PMID: 1594631
9. ICAM-1-mediated, Src- and Pyk2-dependent vascular endothelial cadherin tyrosine phosphorylation is required for leukocyte transendothelial migration.  |  Allingham, MJ., et al. 2007. J Immunol. 179: 4053-64. PMID: 17785844
10. Suppressor of cytokine signaling 2 regulates IL-15-primed human NK cell function via control of phosphorylated Pyk2.  |  Lee, SH., et al. 2010. J Immunol. 185: 917-28. PMID: 20543098
11. Autophosphorylation of the focal adhesion kinase, pp125FAK, directs SH2-dependent binding of pp60src.  |  Schaller, MD., et al. 1994. Mol Cell Biol. 14: 1680-8. PMID: 7509446
12. Protein tyrosine kinase PYK2 involved in Ca(2+)-induced regulation of ion channel and MAP kinase functions.  |  Lev, S., et al. 1995. Nature. 376: 737-45. PMID: 7544443

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-p-PYK2 Antibody (13.Tyr 402) has 3 citations in a variety of scientific publications.

Primary antibodies like Anti-p-PYK2 Antibody (13.Tyr 402) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

p-PYK2 Antibody (13.Tyr 402) Specifications

Alternate Product Name: PYK2

Product Clone Name: 13.Tyr 402

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: Tyr 402

Epitope Target: PYK2

Epitope Species: human

Product Reactivity/Detection: Tyr 402 phosphorylated PYK2

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 3 citations in various scientific publications

Available Conjugates/Forms for p-PYK2 Antibody (13.Tyr 402): AC: Agarose, HRP: Horseradish Peroxidase