p-PKC theta Antibody (A-11): sc-271922

p-PKC θ Antibody (A-11) is a mouse monoclonal IgM antibody that detects p-PKC θ in mouse, rat, and human samples by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). p-PKC θ (A-11) antibody is available in a non-conjugated form, providing flexibility for various experimental needs. p-PKC θ, a member of the protein kinase C (PKC) family, plays a critical role in regulating numerous cellular functions, including cell growth, differentiation, and immune responses. Located primarily in the cytoplasm and translocating to the membrane upon activation, p-PKC θ is essential for T cell receptor signaling and the subsequent activation of downstream pathways, such as NFκB, which is vital for immune response regulation. Proper localization of p-PKC θ is crucial, as membrane association is necessary for activation and function in signaling cascades. Dysregulation of p-PKC θ has been implicated in various diseases, including autoimmune disorders and cancer, highlighting importance as a therapeutic target. Anti-p-PKC θ antibody (A-11) recognizes the phosphorylated form at Thr 538, allowing for the study of activation state and providing insights into signaling mechanisms that govern cellular responses.

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p-PKC theta Antibody (A-11) References:

1. Mammalian TOR controls one of two kinase pathways acting upon nPKCdelta and nPKCepsilon.  |  Parekh, D., et al. 1999. J Biol Chem. 274: 34758-64. PMID: 10574945
2. Phosphorylation sites of protein kinase C delta in H2O2-treated cells and its activation by tyrosine kinase in vitro.  |  Konishi, H., et al. 2001. Proc Natl Acad Sci U S A. 98: 6587-92. PMID: 11381116
3. Characterization of TCR-induced phosphorylation of PKCtheta in primary murine lymphocytes.  |  Thebault, S. and Ochoa-Garay, J. 2004. Mol Immunol. 40: 931-42. PMID: 14725789
4. A new member of the protein kinase C family, nPKC theta, predominantly expressed in skeletal muscle.  |  Osada, S., et al. 1992. Mol Cell Biol. 12: 3930-8. PMID: 1508194
5. Calcium-dependent activation of a multifunctional protein kinase by membrane phospholipids.  |  Takai, Y., et al. 1979. J Biol Chem. 254: 3692-5. PMID: 438153
6. Turnover of inositol phospholipids and signal transduction.  |  Nishizuka, Y. 1984. Science. 225: 1365-70. PMID: 6147898
7. The role of protein kinase C in cell surface signal transduction and tumour promotion.  |  Nishizuka, Y. Nature. 308: 693-8. PMID: 6232463
8. Protein kinase C as a possible receptor protein of tumor-promoting phorbol esters.  |  Kikkawa, U., et al. 1983. J Biol Chem. 258: 11442-5. PMID: 6311812
9. Direct activation of calcium-activated, phospholipid-dependent protein kinase by tumor-promoting phorbol esters.  |  Castagna, M., et al. 1982. J Biol Chem. 257: 7847-51. PMID: 7085651
10. Activation of protein kinase C by tyrosine phosphorylation in response to H2O2.  |  Konishi, H., et al. 1997. Proc Natl Acad Sci U S A. 94: 11233-7. PMID: 9326592

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-p-PKC theta Antibody (A-11) has 4 citations in a variety of scientific publications.

Primary antibodies like Anti-p-PKC theta Antibody (A-11) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

p-PKC theta Antibody (A-11) Specifications

Alternate Product Name: PKC theta

Product Clone Name: A-11

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgM kappa light chain

Amino Acids: Thr 538

Epitope Target: PKC θ

Epitope Species: mouse

Product Reactivity/Detection: Thr 538 phosphorylated p-PKC θ

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgM kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 4 citations in various scientific publications

Available Conjugates/Forms for p-PKC theta Antibody (A-11): P: Blocking Peptide

The preferred PKC antibody is PKC Antibody (A-3): sc-17769.