p-PKA IIβ reg Antibody (pS114.20A): sc-293036

p-PKA IIβ reg Antibody (pS114.20A) is a mouse monoclonal IgG1 kappa light chain antibody that detects Ser 114 phosphorylated PKA IIβ reg of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), and immunofluorescence (IF). p-PKA IIβ reg (pS114.20A) antibody is available in both conjugated and non-conjugated forms, providing flexibility for various experimental needs. p-PKA IIβ reg, also known as PRKAR2B, is a crucial regulatory subunit of the cAMP-dependent protein kinase (PKA) and consists of 418 amino acids. p-PKA IIβ reg functions to modulate PKA activity, which plays a vital role in mediating cellular responses to external signals through the second messenger cyclic AMP. When cAMP binds to regulatory subunits of the PKA holoenzyme, active catalytic subunits are released, thereby influencing processes such as metabolism, gene transcription, and ion transport. p-PKA IIβ reg suppresses CREB transcriptional activity and down-regulates interleukin-2 production in T-lymphocytes, highlighting significance in both metabolic regulation and immune function. Studies on p-PKA IIβ reg knockout mice have revealed that absence of this protein leads to reduced white adipose tissue and protection against diet-induced obesity and fatty liver, further underscoring importance in metabolic health.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

p-PKA IIβ reg Antibody (pS114.20A) References:

1. Mapping of the regulatory subunits RI beta and RII beta of cAMP-dependent protein kinase genes on human chromosome 7.  |  Solberg, R., et al. 1992. Genomics. 14: 63-9. PMID: 1358799
2. Down-regulation of IL-2 production in T lymphocytes by phosphorylated protein kinase A-RIIbeta.  |  Elliott, MR., et al. 2004. J Immunol. 172: 7804-12. PMID: 15187164
3. Requirement for the RIIbeta isoform of PKA, but not calcium-stimulated adenylyl cyclase, in visual cortical plasticity.  |  Fischer, QS., et al. 2004. J Neurosci. 24: 9049-58. PMID: 15483123
4. Barrel map development relies on protein kinase A regulatory subunit II beta-mediated cAMP signaling.  |  Inan, M., et al. 2006. J Neurosci. 26: 4338-49. PMID: 16624954
5. Disruption of the RIIbeta subunit of PKA reverses the obesity syndrome of Agouti lethal yellow mice.  |  Czyzyk, TA., et al. 2008. Proc Natl Acad Sci U S A. 105: 276-81. PMID: 18172198
6. Identification of a clinically homogenous subgroup of benign cortisol-secreting adrenocortical tumors characterized by alterations of the protein kinase A (PKA) subunits and high PKA activity.  |  Vincent-Dejean, C., et al. 2008. Eur J Endocrinol. 158: 829-39. PMID: 18505904
7. Current insights into the role of PKA phosphorylation in CFTR channel activity and the pharmacological rescue of cystic fibrosis disease-causing mutants.  |  Chin, S., et al. 2017. Cell Mol Life Sci. 74: 57-66. PMID: 27722768
8. Molecular cloning, complementary deoxyribonucleic acid structure and predicted full-length amino acid sequence of the hormone-inducible regulatory subunit of 3'-5'-cyclic adenosine monophosphate-dependent protein kinase from human testis.  |  Levy, FO., et al. 1988. Mol Endocrinol. 2: 1364-73. PMID: 2851102
9. BNIP3L/Nix-induced mitochondrial fission, mitophagy, and impaired myocyte glucose uptake are abrogated by PRKA/PKA phosphorylation.  |  da Silva Rosa, SC., et al. 2021. Autophagy. 17: 2257-2272. PMID: 33044904
10. A human regulatory subunit of type II cAMP-dependent protein kinase localized by its linkage relationship to several cloned chromosome 7q markers.  |  Wainwright, B., et al. 1987. Cytogenet Cell Genet. 45: 237-9. PMID: 3691190
11. Targeting the IKs Channel PKA Phosphorylation Axis to Restore Its Function in High-Risk LQT1 Variants.  |  Zhong, L., et al. 2024. Circ Res. 135: 722-738. PMID: 39166328
12. Loss of haloperidol induced gene expression and catalepsy in protein kinase A-deficient mice.  |  Adams, MR., et al. 1997. Proc Natl Acad Sci U S A. 94: 12157-61. PMID: 9342379

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-p-PKA IIβ reg Antibody (pS114.20A) has 3 citations in a variety of scientific publications.

Primary antibodies like Anti-p-PKA IIβ reg Antibody (pS114.20A) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

p-PKA IIβ reg Antibody (pS114.20A) Specifications

Alternate Product Name: PKA IIβ reg

Product Clone Name: pS114.20A

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: Ser 114

Epitope Target: PKA IIβ reg

Epitope Species: human

Product Reactivity/Detection: Ser 114 phosphorylated PKA IIβ reg of human origin and correspondingly Ser 112 phosphorylated PKA IIβ reg of mouse and rat origin

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 3 citations in various scientific publications

Available Conjugates/Forms for p-PKA IIβ reg Antibody (pS114.20A): AC: Agarose, HRP: Horseradish Peroxidase

The preferred PKA IIβ reg antibody is PKA IIβ reg Antibody (C-2): sc-376778.