PKA IIβ reg Antibody (C-2): sc-376778

PKA IIβ reg Antibody (C-2) is a mouse monoclonal IgG3 kappa light chain antibody that detects PKA II beta reg protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-PKA II beta reg antibody (C-2) is available as the non-conjugated form. PKA II beta regulatory protein plays a crucial role in regulating cAMP-dependent protein kinase (PKA) pathway, which mediates various cellular responses to external stimuli, including cell proliferation, ion transport, metabolic regulation, and gene transcription. PKA II beta regulatory protein is primarily located in the cytoplasm, forming a complex with PKA catalytic subunits to control kinase activation in response to elevated cyclic AMP (cAMP) levels. This localization ensures PKA activation occurs only when necessary, preventing inappropriate signaling that could lead to cellular dysfunction. Upon cAMP binding to regulatory subunits, active catalytic subunits release and move to the nucleus, phosphorylating key transcription factors like cAMP response element-binding protein (CREB), influencing gene expression and cellular responses. Precise regulation of PKA activity maintains cellular homeostasis and appropriate physiological responses.

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PKA IIβ reg Antibody (C-2) References:

1. Molecular cloning of a tissue-specific protein kinase (C gamma) from human testis--representing a third isoform for the catalytic subunit of cAMP-dependent protein kinase.  |  Beebe, SJ., et al. 1990. Mol Endocrinol. 4: 465-75. PMID: 2342480
2. Cyclic AMP stimulates somatostatin gene transcription by phosphorylation of CREB at serine 133.  |  Gonzalez, GA. and Montminy, MR. 1989. Cell. 59: 675-80. PMID: 2573431
3. A cDNA clone encoding human cAMP-dependent protein kinase catalytic subunit C alpha.  |  Maldonado, F. and Hanks, SK. 1988. Nucleic Acids Res. 16: 8189-90. PMID: 2843813
4. Cell-type-specific asynchronous modulation of PKA by dopamine in learning.  |  Lee, SJ., et al. 2021. Nature. 590: 451-456. PMID: 33361810
5. PKA compartmentalization links cAMP signaling and autophagy.  |  Grisan, F., et al. 2021. Cell Death Differ. 28: 2436-2449. PMID: 33742135
6. Potential and limitations of PKA/ PKG inhibitors for platelet studies.  |  Shpakova, V., et al. 2022. Platelets. 33: 859-868. PMID: 34845961
7. STK25 inhibits PKA signaling by phosphorylating PRKAR1A.  |  Zhang, X., et al. 2022. Cell Rep. 40: 111203. PMID: 35977512
8. Locomotion activates PKA through dopamine and adenosine in striatal neurons.  |  Ma, L., et al. 2022. Nature. 611: 762-768. PMID: 36352228
9. Promoting proteostasis by cAMP/PKA and cGMP/PKG.  |  Ahammed, MS. and Wang, X. 2024. Trends Mol Med.. PMID: 39477759
10. Activation of protein kinase by physiological concentrations of cyclic AMP.  |  Beavo, JA., et al. 1974. Proc Natl Acad Sci U S A. 71: 3580-3. PMID: 4372627
11. Signal transduction through the cAMP-dependent protein kinase.  |  Meinkoth, JL., et al. 1993. Mol Cell Biochem. 127-128: 179-86. PMID: 7935349
12. Phosphorylation-dephosphorylation of enzymes.  |  Krebs, EG. and Beavo, JA. 1979. Annu Rev Biochem. 48: 923-59. PMID: 38740

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-PKA IIβ reg Antibody (C-2) has 14 citations in a variety of scientific publications.

Primary antibodies like Anti-PKA IIβ reg Antibody (C-2) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

PKA IIβ reg Antibody (C-2) Specifications

Alternate Product Name: PKA IIβ reg

Product Clone Name: C-2

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₃ kappa light chain

Amino Acids: 21-110

Epitope Target: PKA IIβ regulatory chain

Epitope Species: human

Product Reactivity/Detection: PKA IIβ reg

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₃ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 14 citations in various scientific publications

Available Conjugates/Forms for PKA IIβ reg Antibody (C-2): N/A

The preferred p-PKA IIβ reg antibody is p-PKA IIβ reg Antibody (pS114.20A): sc-293036.