p-p70 S6 kinase/S6K1 Antibody (E-5): sc-377529

p-p70 S6 kinase α Antibody (E-5) is a mouse monoclonal IgG1 kappa light chain antibody that detects p-p70 S6 kinase α of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). p-p70 S6 kinase α (E-5) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. p70 S6 kinase α plays a crucial role in regulating protein synthesis and cell growth, acting as a key downstream effector in the mammalian target of rapamycin (mTOR) signaling pathway. This pathway is vital for cellular responses to nutrients and growth factors, and dysregulation is often implicated in various diseases, including cancer and metabolic disorders. Phosphorylation of p70 S6 kinase α at Thr 421 and Ser 424 is essential for activation, which promotes the translation of mRNAs encoding ribosomal proteins and translation factors, thereby enhancing protein synthesis. Understanding p-p70 S6 kinase α function and regulatory mechanisms is important for developing therapeutic strategies targeting mTOR signaling in disease contexts.

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p-p70 S6 kinase/S6K1 Antibody (E-5) References:

1. Immunopurified mammalian target of rapamycin phosphorylates and activates p70 S6 kinase alpha in vitro.  |  Isotani, S., et al. 1999. J Biol Chem. 274: 34493-8. PMID: 10567431
2. Carboxyl-terminal region conserved among phosphoinositide-kinase-related kinases is indispensable for mTOR function in vivo and in vitro.  |  Takahashi, T., et al. 2000. Genes Cells. 5: 765-75. PMID: 10971657
3. Identification of the NIMA family kinases NEK6/7 as regulators of the p70 ribosomal S6 kinase.  |  Belham, C., et al. 2001. Curr Biol. 11: 1155-67. PMID: 11516946
4. Distinct regulatory mechanism for p70 S6 kinase beta from that for p70 S6 kinase alpha.  |  Minami, T., et al. 2001. Genes Cells. 6: 1003-15. PMID: 11733037
5. Raptor, a binding partner of target of rapamycin (TOR), mediates TOR action.  |  Hara, K., et al. 2002. Cell. 110: 177-89. PMID: 12150926
6. Raptor, a binding partner of target of rapamycin.  |  Yonezawa, K., et al. 2004. Biochem Biophys Res Commun. 313: 437-41. PMID: 14684181
7. mTOR integrates amino acid- and energy-sensing pathways.  |  Tokunaga, C., et al. 2004. Biochem Biophys Res Commun. 313: 443-6. PMID: 14684182
8. Cloning and expression of two human p70 S6 kinase polypeptides differing only at their amino termini.  |  Grove, JR., et al. 1991. Mol Cell Biol. 11: 5541-50. PMID: 1922062
9. p70 S6 kinase limits tumor necrosis factor-alpha-induced interleukin-6 synthesis in osteoblast-like cells.  |  Minamitani, C., et al. 2010. Mol Cell Endocrinol. 315: 195-200. PMID: 19879324
10. (-)-Epicatechin treatment modify the expression of genes related to atrophy in gastrocnemius muscle of male rats obese by programing.  |  Alvarez-Chávez, AL., et al. 2024. J Dev Orig Health Dis. 15: e21. PMID: 39370974

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-p-p70 S6 kinase/S6K1 Antibody (E-5) has 7 citations in a variety of scientific publications.

Primary antibodies like Anti-p-p70 S6 kinase/S6K1 Antibody (E-5) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

p-p70 S6 kinase/S6K1 Antibody (E-5) Specifications

Alternate Product Name: p70S6K

Product Clone Name: E-5

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: Thr 421/Ser 424

Epitope Target: p70 S6 kinase α

Epitope Species: human

Product Reactivity/Detection: Thr 421 and Ser 424 dually phosphorylated p70 S6 kinase α

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: equine, canine, bovine, porcine and avian

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 7 citations in various scientific publications

Available Conjugates/Forms for p-p70 S6 kinase/S6K1 Antibody (E-5): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin

The preferred p70 S6 kinase/S6K1 antibody is p70 S6 kinase/S6K1 Antibody (H-9): sc-8418.