p-Waf1/Cip1/CDKN1A p21 Antibody (D-4): sc-377515

p-p21 Waf1/Cip1 Antibody (D-4) is a mouse monoclonal IgG3 kappa light chain antibody that detects Ser 146 phosphorylated p21 Waf1/Cip1 in mouse, rat, and human samples through western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). p-p21 Waf1/Cip1 (D-4) antibody is available in a non-conjugated form, allowing for versatile experimental design. p21 Waf1/Cip1 plays a crucial role in cell cycle regulation by acting as a potent inhibitor of cyclin-dependent kinases (Cdks), which are essential for the transition between different phases of the cell cycle. By binding tightly to Cdks, p21 Waf1/Cip1 prevents retinoblastoma protein (Rb) phosphorylation by various cyclin-Cdk complexes, halting cell cycle progression and promoting cellular senescence. p21 Waf1/Cip1 expression is tightly regulated and induced by wild-type p53, linking p21 Waf1/Cip1 to cellular response to DNA damage and stress. This regulatory mechanism maintains genomic stability and prevents uncontrolled cell proliferation, a hallmark of cancer. The mouse homolog of p21 Waf1/Cip1, known as CAP20, underscores the evolutionary conservation of this regulatory protein. anti-p-p21 Waf1/Cip1 antibody (D-4) serves as a valuable resource for investigating phosphorylation-dependent functions of p21 Waf1/Cip1 in cell cycle regulation and stress response pathways.

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p-Waf1/Cip1/CDKN1A p21 Antibody (D-4) References:

1. Apoptosis inhibitory activity of cytoplasmic p21(Cip1/WAF1) in monocytic differentiation.  |  Asada, M., et al. 1999. EMBO J. 18: 1223-34. PMID: 10064589
2. Reversible phosphorylation at the C-terminal regulatory domain of p21(Waf1/Cip1) modulates proliferating cell nuclear antigen binding.  |  Scott, MT., et al. 2000. J Biol Chem. 275: 11529-37. PMID: 10753973
3. Glycogen synthase kinase-3 couples AKT-dependent signaling to the regulation of p21Cip1 degradation.  |  Rössig, L., et al. 2002. J Biol Chem. 277: 9684-9. PMID: 11779850
4. Phosphorylated Pak1 level in the cytoplasm correlates with shorter survival time in patients with glioblastoma.  |  Aoki, H., et al. 2007. Clin Cancer Res. 13: 6603-9. PMID: 18006760
5. Inhibition of PP2A by LB100 sensitizes bladder cancer cells to chemotherapy by inducing p21 degradation.  |  Gao, S., et al. 2022. Cell Oncol (Dordr). 45: 1203-1215. PMID: 36136269
6. Cell-cycle inhibition by independent CDK and PCNA binding domains in p21Cip1.  |  Luo, Y., et al. 1995. Nature. 375: 159-61. PMID: 7753174
7. The p21 Cdk-interacting protein Cip1 is a potent inhibitor of G1 cyclin-dependent kinases.  |  Harper, JW., et al. 1993. Cell. 75: 805-16. PMID: 8242751
8. WAF1, a potential mediator of p53 tumor suppression.  |  el-Deiry, WS., et al. 1993. Cell. 75: 817-25. PMID: 8242752
9. p21(Waf1/Cip1) protects against p53-mediated apoptosis of human melanoma cells.  |  Gorospe, M., et al. 1997. Oncogene. 14: 929-35. PMID: 9050992
10. Interaction with cyclin-dependent kinases and PCNA modulates proteasome-dependent degradation of p21.  |  Cayrol, C. and Ducommun, B. 1998. Oncogene. 17: 2437-44. PMID: 9824154

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-p-Waf1/Cip1/CDKN1A p21 Antibody (D-4) has 4 citations in a variety of scientific publications.

Primary antibodies like Anti-p-Waf1/Cip1/CDKN1A p21 Antibody (D-4) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

p-Waf1/Cip1/CDKN1A p21 Antibody (D-4) Specifications

Alternate Product Name: p21 Waf1/Cip1

Product Clone Name: D-4

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₃ kappa light chain

Amino Acids: Ser 146

Epitope Target: p21 Waf1/Cip1

Epitope Species: human

Product Reactivity/Detection: Ser 146 phosphorylated p21 Waf1/Cip1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: canine, bovine and porcine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₃ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 4 citations in various scientific publications

Available Conjugates/Forms for p-Waf1/Cip1/CDKN1A p21 Antibody (D-4): P: Blocking Peptide

The preferred Waf1/Cip1/CDKN1A p21 antibody is Waf1/Cip1/CDKN1A p21 Antibody (F-5): sc-6246.