p-MEK-1/2 Antibody (7E10): sc-81503

p-MEK-1/2 Antibody (7E10) is a mouse monoclonal IgG1 kappa light chain antibody that detects p-MEK-1/2 in mouse, rat, human, and canine samples through western blotting (WB) and immunoprecipitation (IP). anti-p-MEK-1/2 antibody (7E10) is available in a non-conjugated form, allowing for versatile applications in various experimental setups. p-MEK-1/2 (7E10) antibody recognizes a protein that plays a crucial role in the MAPK signaling pathway, which is essential for regulating cell proliferation, differentiation, and survival. Specifically, p-MEK-1 and p-MEK-2 are dual-specificity kinases that phosphorylate and activate extracellular signal-regulated kinases (ERKs), which are pivotal in mediating cellular responses to growth factors and stress signals. The phosphorylation of MEK-1 and MEK-2 at specific serine residues (Ser 218 and 222 for MEK-1, and Ser 222 and 226 for MEK-2) is a key regulatory mechanism that enhances their kinase activity, thereby influencing downstream signaling cascades. Understanding the function of p-MEK-1/2 is vital, as dysregulation of this pathway is implicated in various diseases, including cancer, making p-MEK-1/2 a significant target for therapeutic intervention.

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p-MEK-1/2 Antibody (7E10) References:

1. MEK-1/2 inhibition prevents 5-lipoxygenase translocation in N-formylpeptide-challenged human neutrophils.  |  Boden, SE., et al. 2000. Int J Biochem Cell Biol. 32: 1069-74. PMID: 11091139
2. MEK-1/2 inhibition reduces branching morphogenesis and causes mesenchymal cell apoptosis in fetal rat lungs.  |  Kling, DE., et al. 2002. Am J Physiol Lung Cell Mol Physiol. 282: L370-8. PMID: 11839529
3. Importance of MEK-1/-2 signaling in monocytic and granulocytic differentiation of myeloid cell lines.  |  Miranda, MB., et al. 2002. Leukemia. 16: 683-92. PMID: 11960350
4. Activation of MEK 1/2 and p42/44 MAPK by angiotensin II in hepatocyte nucleus and their potentiation by ethanol.  |  Aroor, AR., et al. 2009. Alcohol. 43: 315-22. PMID: 19560630
5. A phase II evaluation of selumetinib (AZD6244, ARRY-142886), a selective MEK-1/2 inhibitor in the treatment of recurrent or persistent endometrial cancer: an NRG Oncology/Gynecologic Oncology Group study.  |  Coleman, RL., et al. 2015. Gynecol Oncol. 138: 30-5. PMID: 25887099
6. MEK 1/2 inhibitors in the treatment of hepatocellular carcinoma.  |  Facciorusso, A., et al. 2015. Expert Rev Gastroenterol Hepatol. 9: 993-1003. PMID: 25915713
7. Orexin-A protects SH-SY5Y cells against H2O2-induced oxidative damage via the PI3K/MEK1/2/ERK1/2 signaling pathway.  |  Wang, CM., et al. 2018. Int J Immunopathol Pharmacol. 32: 2058738418785739. PMID: 29983082
8. Oral MEK 1/2 Inhibitor Trametinib in Combination With AKT Inhibitor GSK2141795 in Patients With Acute Myeloid Leukemia With RAS Mutations: A Phase II Study.  |  Ragon, BK., et al. 2019. Clin Lymphoma Myeloma Leuk. 19: 431-440.e13. PMID: 31056348
9. Genistein regulates calcium and phosphate homeostasis without activation of MEK 1/2 signalling pathway in an animal model of the andropause.  |  Živanović, J., et al. 2022. Ann Anat. 239: 151836. PMID: 34563672
10. The MEK 1/2 inhibitor PD98059 exhibits synergistic anti-endometrial cancer activity with paclitaxel in vitro and enhanced tissue distribution in vivo when formulated into PAMAM-coated PLGA-PEG nanoparticles.  |  Wiwatchaitawee, K., et al. 2022. Drug Deliv Transl Res. 12: 1684-1696. PMID: 34635984

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-p-MEK-1/2 Antibody (7E10) has 44 citations in a variety of scientific publications.

Primary antibodies like Anti-p-MEK-1/2 Antibody (7E10) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

p-MEK-1/2 Antibody (7E10) Specifications

Alternate Product Name: MAP2K1, MAP2K2

Product Clone Name: 7E10

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: Ser 218/222

Epitope Target: MEK-1/2

Epitope Species: human

Product Reactivity/Detection: Ser 218 and 222 phosphorylated MEK-1 and Ser 222 and 226 phosphorylated MEK-2

Species Reactivity/Detection: mouse, rat, human, canine

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP

Vialing Information: Each vial contains 50 µg IgG₁ kappa light chain in 0.5 ml of PBS with < 0.1% sodium azide, 0.1% gelatin, PEG and sucrose

Research Citations: 44 citations in various scientific publications

Available Conjugates/Forms for p-MEK-1/2 Antibody (7E10): N/A

The preferred MEK-1 antibody is MEK-1 Antibody (H-8): sc-6250.