p-JNK Antibody (G-7): sc-6254

p-JNK Antibody (G-7) is a mouse monoclonal IgG1 kappa light chain antibody raised against phosphorylated human c-Jun N-terminal kinase (p-JNK), a stress-activated protein kinase crucial for cellular stress responses. p-JNK mouse monoclonal antibody (G-7) recognizes JNK when phosphorylated and demonstrates broad species cross-reactivity, detecting p-JNK in mouse, rat, and human samples through Western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry (IHCP), and enzyme-linked immunosorbent assay (ELISA) applications. Anti-p-JNK monoclonal antibody (G-7) is available in non-conjugated form and multiple conjugated forms including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and various Alexa Fluor® conjugates, offering versatility for diverse experimental needs. The JNK family encompasses three members - JNK1, JNK2, and JNK3 - which undergo activation through a sophisticated dual phosphorylation mechanism at Thr183 and Tyr185. These specific phosphorylation events, catalyzed by JNK-activating kinases within the mitogen-activated protein kinase (MAPK) cascade, enable JNK to respond effectively to various cellular stressors, particularly ultraviolet light exposure. Once activated, p-JNK phosphorylates c-Jun at Ser63 and Ser73, enhancing c-Jun′s ability to regulate gene expression. This phosphorylation cascade proves essential for controlling cellular processes including proliferation, differentiation, and apoptosis in response to stress and inflammatory signals. Through these diverse regulatory roles, p-JNK signaling significantly influences both normal cellular functions and the development of various pathological conditions, including cancer and neurodegenerative disorders. p-JNK (G-7) antibody serves as a valuable tool for investigating these complex signaling pathways and their implications in disease mechanisms.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

p-JNK Antibody (G-7) References:

1. The JNKK2-JNK1 fusion protein acts as a constitutively active c-Jun kinase that stimulates c-Jun transcription activity.  |  Zheng, C., et al. 1999. J Biol Chem. 274: 28966-71. PMID: 10506143
2. Oncoprotein-mediated signalling cascade stimulates c-Jun activity by phosphorylation of serines 63 and 73.  |  Smeal, T., et al. 1992. Mol Cell Biol. 12: 3507-13. PMID: 1630458
3. Pro-Leu-Ser/Thr-Pro is a consensus primary sequence for substrate protein phosphorylation. Characterization of the phosphorylation of c-myc and c-jun proteins by an epidermal growth factor receptor threonine 669 protein kinase.  |  Alvarez, E., et al. 1991. J Biol Chem. 266: 15277-85. PMID: 1651323
4. Phosphorylation of c-jun mediated by MAP kinases.  |  Pulverer, BJ., et al. 1991. Nature. 353: 670-4. PMID: 1922387
5. ERKs: a family of protein-serine/threonine kinases that are activated and tyrosine phosphorylated in response to insulin and NGF.  |  Boulton, TG., et al. 1991. Cell. 65: 663-75. PMID: 2032290
6. Evaluation of the p-AKT, p-JNK and FoxO3a function in oral epithelial dysplasia.  |  Chaves, FN., et al. 2017. Oral Dis. 23: 367-378. PMID: 27976455
7. Inhibition of p-mTOR represses PS1 transcription by reducing p-JNK.  |  Das, HK. and Hontiveros, SS. 2020. Front Biosci (Landmark Ed). 25: 1297-1304. PMID: 32114433
8. Neuroprotective mechanism of salvianolic acid B against cerebral ischemia-reperfusion injury in mice through downregulation of TLR4, p-p38MAPK, p-JNK, NF-κB, and IL-1β.  |  Zheng, XF., et al. 2023. Immun Inflamm Dis. 11: e1030. PMID: 37904689
9. JNK1: a protein kinase stimulated by UV light and Ha-Ras that binds and phosphorylates the c-Jun activation domain.  |  Dérijard, B., et al. 1994. Cell. 76: 1025-37. PMID: 8137421
10. The stress-activated protein kinase subfamily of c-Jun kinases.  |  Kyriakis, JM., et al. 1994. Nature. 369: 156-60. PMID: 8177321

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-p-JNK Antibody (G-7) has 1,454 citations in a variety of scientific publications.

Primary antibodies like Anti-p-JNK Antibody (G-7) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

p-JNK Antibody (G-7) Specifications

Alternate Product Name: JNK

Product Clone Name: G-7

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: Thr 183/Tyr 185

Epitope Target: JNK

Epitope Species: human

Product Reactivity/Detection: Thr 183 and Tyr 185 phosphorylated JNK1, JNK2 and JNK3

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1,454 citations in various scientific publications

Available Conjugates/Forms for p-JNK Antibody (G-7): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, B: Biotin, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin, TRITC: Rhodamine