p-JNK Antibody (14.Thr 183/Tyr 185): sc-293136

p-JNK Antibody (14.Thr183/Tyr185) is a mouse monoclonal IgG1 kappa light chain antibody raised against human JNK phosphorylated at both Thr183 and Tyr185, key regulatory sites of this stress-activated protein kinase. p-JNK mouse monoclonal antibody (14.Thr183/Tyr185) specifically recognizes JNK only when both threonine 183 and tyrosine 185 are phosphorylated and demonstrates broad species cross-reactivity, detecting phosphorylated JNK in mouse, rat, and human samples through Western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA) applications. Anti-p-JNK monoclonal antibody (14.Thr183/Tyr185) is available in both conjugated and non-conjugated forms, offering versatility for diverse experimental needs. The JNK family members (JNK1, JNK2, and JNK3) function as critical components within the mitogen-activated protein kinase (MAPK) cascade, where dual phosphorylation serves as a molecular switch controlling their activation. JNK2 and JNK3, comprising 424 and 464 amino acids respectively, maintain dynamic distribution between cytoplasmic and nuclear compartments. This spatial regulation enables JNKs to respond rapidly to various activating signals, including cytokines and growth factors. The phosphorylation at Thr183 and Tyr185 proves particularly crucial as these modifications trigger JNK′s catalytic activity, enabling phosphorylation of downstream targets that control stress responses, apoptosis, and inflammation. The significance of precise JNK regulation becomes especially evident with JNK3, where mutations can lead to severe neurological conditions such as Lennox-Gastaut syndrome. Through these phosphorylation-dependent functions, p-JNK (14.Thr183/Tyr185) antibody serves as a valuable tool for investigating both normal signaling mechanisms and disease pathways.

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Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-p-JNK Antibody (14.Thr 183/Tyr 185) has 65 citations in a variety of scientific publications.

Primary antibodies like Anti-p-JNK Antibody (14.Thr 183/Tyr 185) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

p-JNK Antibody (14.Thr 183/Tyr 185) Specifications

Alternate Product Name: JNK

Product Clone Name: 14.Thr 183/Tyr 185

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: Thr 183/Tyr 185

Epitope Target: JNK

Epitope Species: human

Product Reactivity/Detection: Thr 183 and Tyr 185 phosphorylated JNK1, and correspondingly phosphorylated JNK2 and JNK3

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 65 citations in various scientific publications

Available Conjugates/Forms for p-JNK Antibody (14.Thr 183/Tyr 185): AC: Agarose, HRP: Horseradish Peroxidase

The preferred p-JNK antibody is p-JNK Antibody (G-7): sc-6254.