Every item is shipped based on the best shipping method assessed for the temperature requirements of that specific item. Items are grouped and shipped together whenever
possible, and a separate shipping charge will be included for each shipping method required. Shipping charges listed below are from our US warehouses to the Contiguous US,
Alaska, Hawaii, Canada and Puerto Rico. Shipping charges for countries outside the US and Canada will be determined once order has been received
Please note: We can not ship to PO boxes
Express Blue Ice
Express Dry Ice
Animal Health Prescription Item
SHIPPING METHODS & CHARGES
Ships via FedEx Ground to Contiguous US, Alaska, Canada, Monday through Friday. This method is used for less temperature sensitive items such as lab ware and animal
health products, bulky and/or heavy items
Labware ships FedEx Ground free of charge to the contiguous US
HSP 27 is a constitutively expressed cytoplasmic protein that co-localizes to the nucleus upon stress-induced insult. Heat shock, cytokines and hormones are among the factors that stimulate the synthesis of HSP 27. The intracellular concentration of the mammalian heat shock protein, HSP 27, increases several-fold after heat shock and other metabolic stresses, and is closely associated with the acquisition of thermotolerance. MAP kinase-activated protein kinase-2 phosphorylates HSP 27 on Serine residues Ser 15, Ser 78 and Ser 82, which are phosphorylated in vivo in response to growth factors and heat shock. Ser 15, Ser 78 and Ser 82 occur in the sequence motif RXXS, which is recognized by ribosomal protein S6 kinase II.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
I am using p-HSP 27 (5B9): sc-81498 antibody for immunoprecipitation, how much antibody should I use?
Asked by: jerojero
Thank you for your question. We recommend using 1–10 µl (i.e., 0.2–2 µg) of antibody with 1 ml of cell lysate. However, optimal antibody concentration should be determined by titration. Please find our full immunoprecipitation protocol here:
Rated 4 out of
Reduction in signal supporting specificityReduction in signal supporting specificity via western blot analysis of HSP 27 phosphorylation in serum starved Hep G2 cells and serum starved Hep G2 cells treated with 10 ng/ml EGF for 5 min, 15 min, 30 min, 1 hr, 2 hrs, 4 hrs and 8 hrs. -SCBT QC
Date published: 2015-03-22
Rated 4 out of
Supporting western blot analysis of HSP 27Supporting western blot analysis of HSP 27 phosphorylation in non-transfected 293T, mouse HSP 27 transfected 293T and ECV304 whole cell lysates. -SCBT QC
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