p-eEF2K Antibody (H-2): sc-377536
- p-eEF2K Antibody (H-2) is a mouse monoclonal IgG2b κ provided at 200 µg/ml
- Anti-p-eEF2K Antibody (H-2) is recommended for detection of Ser 366 phosphorylated eEF2K of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including and equine, canine, bovine and porcine
- Anti-p-eEF2K Antibody (H-2) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for p-eEF2K Antibody (H-2). This work is in progress.
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SEE ALSO...
p-eEF2K Antibody (H-2) is a mouse monoclonal IgG2b kappa light chain antibody that detects Ser 366 phosphorylated eEF2K in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). p-eEF2K (H-2) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. The eukaryotic elongation-factor-2 kinase (eEF2K) plays a crucial role in regulating protein synthesis by phosphorylating and inactivating eEF2, thereby modulating the rate of polypeptide chain elongation during translation. This regulation is particularly important in response to cellular stress and growth signals, as eEF2K activity is tightly controlled by calcium and calmodulin, with autophosphorylation occurring on serine and threonine residues. Notably, eEF2K is phosphorylated at Ser 366 by p70 S6 kinase, which leads to inactivation, especially under low calcium conditions, highlighting eEF2K′s role in cellular adaptation to nutrient availability and stress. The phosphorylation of eEF2K at Ser 359, which is closely located to Ser 366, is also influenced by insulin-like growth factor-1, further illustrating the intricate regulatory mechanisms governing eEF2K activity and significance in cellular physiology.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
p-eEF2K Antibody (H-2) References:
- Mapping the functional domains of elongation factor-2 kinase. | Pavur, KS., et al. 2000. Biochemistry. 39: 12216-24. PMID: 11015200
- Interplay between insulin and nutrients in the regulation of translation factors. | Proud, CG., et al. 2001. Biochem Soc Trans. 29: 541-7. PMID: 11498025
- A novel method to identify protein kinase substrates: eEF2 kinase is phosphorylated and inhibited by SAPK4/p38delta. | Knebel, A., et al. 2001. EMBO J. 20: 4360-9. PMID: 11500363
- Regulation of elongation factor 2 kinase by p90(RSK1) and p70 S6 kinase. | Wang, X., et al. 2001. EMBO J. 20: 4370-9. PMID: 11500364
- eEF2K protects MYCN-amplified cells from starvation. | Delaidelli, A., et al. 2017. Cell Cycle. 16: 1633-1634. PMID: 28767324
- Eef2k is not required for fertility in male mice. | Feng, T., et al. 2021. Transl Androl Urol. 10: 1988-1999. PMID: 34159079
- Roles of eukaryotic elongation factor 2 kinase (eEF2K) in neuronal plasticity, cognition, and Alzheimer disease. | Ma, T. 2023. J Neurochem. 166: 47-57. PMID: 34796967
- EEF2K silencing inhibits tumour progression through repressing SPP1 and synergises with BET inhibitors in melanoma. | Deng, G., et al. 2022. Clin Transl Med. 12: e722. PMID: 35184394
- eEF2K promotes PD-L1 stabilization through inactivating GSK3β in melanoma. | Chen, X., et al. 2022. J Immunother Cancer. 10: PMID: 35347072
- eEF2K Inhibitor Design: The Progression of Exemplary Structure-Based Drug Design. | Klupt, KA. and Jia, Z. 2023. Molecules. 28: PMID: 36770760
- Design and Characterization of a Novel eEF2K Degrader with Potent Therapeutic Efficacy Against Triple-Negative Breast Cancer. | Zhong, C., et al. 2024. Adv Sci (Weinh). 11: e2305035. PMID: 38084501
- Purification and phosphorylation of elongation factor-2 kinase from rabbit reticulocytes. | Redpath, NT. and Proud, CG. 1993. Eur J Biochem. 212: 511-20. PMID: 8444188
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
p-eEF2K Antibody (H-2) | sc-377536 | 200 µg/ml | $316.00 | |||
p-eEF2K Antibody (H-2) AC | sc-377536 AC | 500 µg/ml, 25% agarose | $416.00 | |||
p-eEF2K Antibody (H-2) HRP | sc-377536 HRP | 200 µg/ml | $316.00 | |||
p-eEF2K Antibody (H-2) FITC | sc-377536 FITC | 200 µg/ml | $330.00 | |||
p-eEF2K Antibody (H-2) PE | sc-377536 PE | 200 µg/ml | $343.00 | |||
p-eEF2K Antibody (H-2) Alexa Fluor® 488 | sc-377536 AF488 | 200 µg/ml | $357.00 | |||
p-eEF2K Antibody (H-2) Alexa Fluor® 546 | sc-377536 AF546 | 200 µg/ml | $357.00 | |||
p-eEF2K Antibody (H-2) Alexa Fluor® 594 | sc-377536 AF594 | 200 µg/ml | $357.00 | |||
p-eEF2K Antibody (H-2) Alexa Fluor® 647 | sc-377536 AF647 | 200 µg/ml | $357.00 | |||
p-eEF2K Antibody (H-2) Alexa Fluor® 680 | sc-377536 AF680 | 200 µg/ml | $357.00 | |||
p-eEF2K Antibody (H-2) Alexa Fluor® 790 | sc-377536 AF790 | 200 µg/ml | $357.00 | |||
p-eEF2K (H-2) Neutralizing Peptide | sc-377536 P | 100 µg/0.5 ml | $68.00 |