p-Dynamin I Antibody (E-9): sc-377563
- p-Dynamin I Antibody (E-9) is a mouse monoclonal IgG1 κ provided at 200 µg/ml
- specific for an epitope mapping between amino acids Ser 795 Ser 795 of Dynamin I of human origin
- recommended for detection of Ser 795 phosphorylated Dynamin I of mouse, rat and human origin by WB, IP, IF and ELISA; also reactive with additional species, including and canine, bovine and porcine
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for p-Dynamin I Antibody (E-9) for WB applications. These reagents are now offered in bundles with p-Dynamin I Antibody (E-9) (see ordering information below).
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p-Dynamin I Antibody (E-9) is a mouse monoclonal IgG1 antibody that detects Dynamin I in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-Dynamin I antibody (E-9) specifically recognizes the phosphorylated form of Dynamin I, a crucial GTPase enzyme that plays a vital role in the retrieval of synaptic vesicles following exocytosis and is essential for endocytosis by promoting the assembly of invaginating synaptic vesicles. Dynamin I (E-9) antibody targets proteins predominantly located in the cytosolic compartment of nerve terminals, where phosphorylation occurs by protein kinase C at a single site, Serine 795. This phosphorylation event influences Dynamin I′s interaction with the SH3 domain of p85, the regulatory subunit of phosphatidylinositol 3-kinase, thereby affecting downstream signaling pathways. Notably, Dynamin I dephosphorylation is necessary for effective synaptic vesicle retrieval, indicating that phosphorylation state is critical for determining subcellular localization and functional activity. Dynamin I monoclonal antibody (E-9) provides valuable insights into the regulatory mechanisms of synaptic transmission, making this antibody an essential tool for researchers studying neuronal function and signaling pathways.
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p-Dynamin I Antibody (E-9) References:
- Phosphorylation of dynamin I on Ser-795 by protein kinase C blocks its association with phospholipids. | Powell, KA., et al. 2000. J Biol Chem. 275: 11610-7. PMID: 10766777
- Cophosphorylation of amphiphysin I and dynamin I by Cdk5 regulates clathrin-mediated endocytosis of synaptic vesicles. | Tomizawa, K., et al. 2003. J Cell Biol. 163: 813-24. PMID: 14623869
- Dynamin I phosphorylation and the control of synaptic vesicle endocytosis. | Smillie, KJ. and Cousin, MA. 2005. Biochem Soc Symp. 87-97. PMID: 15649133
- Syndapin I is the phosphorylation-regulated dynamin I partner in synaptic vesicle endocytosis. | Anggono, V., et al. 2006. Nat Neurosci. 9: 752-60. PMID: 16648848
- Syndapin I and endophilin I bind overlapping proline-rich regions of dynamin I: role in synaptic vesicle endocytosis. | Anggono, V. and Robinson, PJ. 2007. J Neurochem. 102: 931-43. PMID: 17437541
- Dynamin I phosphorylation by GSK3 controls activity-dependent bulk endocytosis of synaptic vesicles. | Clayton, EL., et al. 2010. Nat Neurosci. 13: 845-51. PMID: 20526333
- Phosphorylation of dynamin I and synaptic-vesicle recycling. | Robinson, PJ., et al. 1994. Trends Neurosci. 17: 348-53. PMID: 7526507
- Calcineurin inhibition of dynamin I GTPase activity coupled to nerve terminal depolarization. | Liu, JP., et al. 1994. Science. 265: 970-3. PMID: 8052858
- Dynamin I is a Ca(2+)-sensitive phospholipid-binding protein with very high affinity for protein kinase C. | Liu, JP., et al. 1994. J Biol Chem. 269: 21043-50. PMID: 8063723
- Calcium binds dynamin I and inhibits its GTPase activity. | Liu, JP., et al. 1996. J Neurochem. 66: 2074-81. PMID: 8780038
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
p-Dynamin I Antibody (E-9) | sc-377563 | 200 µg/ml | $322.00 | |||
p-Dynamin I Antibody (E-9): m-IgG Fc BP-HRP Bundle | sc-540736 | 200 µg Ab; 10 µg BP | $361.00 | |||
p-Dynamin I Antibody (E-9): m-IgG1 BP-HRP Bundle | sc-542335 | 200 µg Ab; 20 µg BP | $361.00 | |||
p-Dynamin I (E-9) Neutralizing Peptide | sc-377563 P | 100 µg/0.5 ml | $69.00 |