p-CD3-ζ Antibody (C415.9A): sc-9975

p-CD3-ζ Antibody (C415.9A) is a mouse monoclonal IgG1 kappa light chain antibody that detects p-CD3-zeta protein of human origin by western blotting (WB), immunoprecipitation (IP), flow cytometry (FCM), and enzyme-linked immunosorbent assay (ELISA). anti-p-CD3-ζ antibody (C415.9A) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. p-CD3-zeta protein plays a crucial role in T cell activation as a component of the T cell receptor (TCR) complex, which is essential for the immune response. p-CD3-zeta is located in the plasma membrane of T cells, where p-CD3-zeta forms part of a multisubunit complex that transmits signals upon recognition of foreign antigens. This signaling cascade is vital for the activation of T cells, leading to their proliferation and differentiation into effector cells that can target and eliminate pathogens. The zeta chain contains three immunoreceptor tyrosine-based activation motifs (ITAMs), which are critical for recruiting and activating downstream signaling molecules such as ZAP-70 and Syk. Phosphorylation of these ITAMs is a key step in the signaling process, allowing for the amplification of the immune response. Understanding p-CD3-zeta function and importance is essential for developing therapeutic strategies aimed at modulating T cell activity in various diseases, including cancer and autoimmune disorders.

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p-CD3-ζ Antibody (C415.9A) References:

1. Structure, assembly and intracellular transport of the T cell receptor for antigen.  |  Exley, M., et al. 1991. Semin Immunol. 3: 283-97. PMID: 1686832
2. Signal transduction by the T cell antigen receptor.  |  Weiss, A., et al. 1991. Semin Immunol. 3: 313-24. PMID: 1839225
3. Imaging T-cell receptor activation reveals accumulation of tyrosine-phosphorylated CD3ζ in the endosomal compartment.  |  Yudushkin, IA. and Vale, RD. 2010. Proc Natl Acad Sci U S A. 107: 22128-33. PMID: 21135224
4. TCR crosslinking promotes Crk adaptor protein binding to tyrosine-phosphorylated CD3ζ chain.  |  Dong, G., et al. 2017. Biochem Biophys Res Commun. 488: 541-546. PMID: 28526413
5. CD28 Costimulation Augments CAR Signaling in NK Cells via the LCK/CD3ζ/ZAP70 Signaling Axis.  |  Acharya, S., et al. 2024. Cancer Discov. 14: 1879-1900. PMID: 38900051
6. Specific interaction of the CD45 protein-tyrosine phosphatase with tyrosine-phosphorylated CD3 zeta chain.  |  Furukawa, T., et al. 1994. Proc Natl Acad Sci U S A. 91: 10928-32. PMID: 7526385
7. Signal transduction. Zapping tandem SH2 domains.  |  Weiss, A. 1995. Nature. 377: 17-8. PMID: 7659151
8. Molecular basis for interaction of the protein tyrosine kinase ZAP-70 with the T-cell receptor.  |  Hatada, MH., et al. 1995. Nature. 377: 32-8. PMID: 7659156
9. Binding of ZAP-70 to phosphorylated T-cell receptor zeta and eta enhances its autophosphorylation and generates specific binding sites for SH2 domain-containing proteins.  |  Neumeister, EN., et al. 1995. Mol Cell Biol. 15: 3171-8. PMID: 7760813
10. Different cytoplasmic structure of the CD3 zeta family dimer modulates the activation signal and function of T cells.  |  Aoe, T., et al. 1994. Int Immunol. 6: 1671-9. PMID: 7865460
11. The role of protein tyrosine kinases and protein tyrosine phosphatases in T cell antigen receptor signal transduction.  |  Chan, AC., et al. 1994. Annu Rev Immunol. 12: 555-92. PMID: 8011291
12. Targeted disruption of the CD3 eta locus causes high lethality in mice: modulation of Oct-1 transcription on the opposite strand.  |  Ohno, H., et al. 1994. EMBO J. 13: 1157-65. PMID: 8131747

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-p-CD3-ζ Antibody (C415.9A) has 17 citations in a variety of scientific publications.

Primary antibodies like Anti-p-CD3-ζ Antibody (C415.9A) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

p-CD3-ζ Antibody (C415.9A) Specifications

Alternate Product Name: CD247

Product Clone Name: C415.9A

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 52-163

Epitope Target: CD3-ζ precursor

Epitope Species: human

Product Reactivity/Detection: phosphorylated CD3-ζ

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, FCM, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 17 citations in various scientific publications

Available Conjugates/Forms for p-CD3-ζ Antibody (C415.9A): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred CD3-ζ antibody is CD3-ζ Antibody (6B10.2): sc-1239.