p-Cbl Antibody (E-10): sc-377571

p-Cbl Antibody (E-10) is a mouse monoclonal IgM antibody that detects Tyr 700 phosphorylated Cbl in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). p-Cbl (E-10) antibody is available in a non-conjugated form, allowing for versatile experimental use. Cbl, also known as the c-Cbl proto-oncogene, E3 ubiquitin-protein ligase CBL, and Casitas B-lineage lymphoma proto-oncogene, plays a crucial role in cellular signaling pathways by functioning as an E3 ubiquitin ligase, which is essential for the regulation of protein degradation and cellular homeostasis. Cbl′s function lies in modulating the activity of various signaling proteins, thereby influencing processes such as cell proliferation, differentiation, and survival. Dysregulation of Cbl has been implicated in several hematological malignancies, as Cbl is involved in the ubiquitination and subsequent degradation of proteins that promote oncogenic signaling. c-Cbl gene product is predominantly localized in the cytoplasm, where c-Cbl interacts with multiple signaling molecules, highlighting c-Cbl′s significance in maintaining normal cellular functions and preventing tumorigenesis. Additionally, c-Cbl gene is located on human chromosome 11q23, a region frequently associated with chromosomal translocations in leukemias, further underscoring anti-p-Cbl antibody (E-10)′s relevance in cancer research and diagnostics.

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p-Cbl Antibody (E-10) References:

1. Relationship of the human protooncogene CBL2 on 11q23 to the t(4;11), t(11;22), and t(11;14) breakpoints.  |  Savage, PD., et al. 1991. Cytogenet Cell Genet. 56: 112-5. PMID: 2013228
2. The sequences of the human and mouse c-cbl proto-oncogenes show v-cbl was generated by a large truncation encompassing a proline-rich domain and a leucine zipper-like motif.  |  Blake, TJ., et al. 1991. Oncogene. 6: 653-7. PMID: 2030914
3. CIN85 drives B cell responses by linking BCR signals to the canonical NF-kappaB pathway.  |  Kometani, K., et al. 2011. J Exp Med. 208: 1447-57. PMID: 21708930
4. The c-cbl proto-oncogene is preferentially expressed in thymus and testis tissue and encodes a nuclear protein.  |  Langdon, WY., et al. 1989. J Virol. 63: 5420-4. PMID: 2585608
5. Identification of two murine loci homologous to the v-cbl oncogene.  |  Regnier, DC., et al. 1989. J Virol. 63: 3678-82. PMID: 2760978
6. v-cbl, an oncogene from a dual-recombinant murine retrovirus that induces early B-lineage lymphomas.  |  Langdon, WY., et al. 1989. Proc Natl Acad Sci U S A. 86: 1168-72. PMID: 2784003
7. A General Strategy for Discovery of Inhibitors and Activators of RING and U-box E3 Ligases with Ubiquitin Variants.  |  Gabrielsen, M., et al. 2017. Mol Cell. 68: 456-470.e10. PMID: 29053960
8. Prognostic significance of chromosomal abnormalities in acute leukaemias and myelodysplastic syndromes.  |  Yunis, JJ. and Brunning, RD. 1986. Clin Haematol. 15: 597-620. PMID: 3536236
9. Formation of c-Cbl.phosphatidylinositol 3-kinase complexes on lymphocyte membranes by a p56lck-independent mechanism.  |  Hartley, D. and Corvera, S. 1996. J Biol Chem. 271: 21939-43. PMID: 8702998
10. Interleukin-2 stimulation induces tyrosine phosphorylation of p120-Cbl and CrkL and formation of multimolecular signaling complexes in T lymphocytes and natural killer cells.  |  Gesbert, F., et al. 1998. J Biol Chem. 273: 3986-93. PMID: 9461587

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-p-Cbl Antibody (E-10) has 4 citations in a variety of scientific publications.

Primary antibodies like Anti-p-Cbl Antibody (E-10) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

p-Cbl Antibody (E-10) Specifications

Alternate Product Name: c-Cbl

Product Clone Name: E-10

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgM kappa light chain

Amino Acids: Tyr 700

Epitope Target: Cbl

Epitope Species: human

Product Reactivity/Detection: Tyr 700 phosphorylated Cbl

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: porcine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgM kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 4 citations in various scientific publications

Available Conjugates/Forms for p-Cbl Antibody (E-10): P: Blocking Peptide

The preferred Cbl antibody is Cbl Antibody (A-9): sc-1651.