p-Atm Antibody (10H11.E12): sc-47739

p-Atm Antibody (10H11.E12) is a mouse monoclonal IgG1 kappa light chain antibody that detects Ser 1981 phosphorylated Atm of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry. p-Atm (10H11.E12) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Atm protein plays a crucial role in cellular response to DNA damage, particularly in detecting and repairing double-strand breaks. This function maintains genomic stability, as mutations in Atm gene are associated with ataxia telangiectasia (AT), leading to severe neurological and immunological defects. In response to DNA damage, Atm rapidly activates through autophosphorylation at serine 1981, initiating a cascade of signaling events that lead to cell cycle arrest and DNA repair. Most human tissues predominantly express non-phosphorylated, inactive Atm, highlighting the importance of post-translational modifications in regulating activity and function. anti-Atm antibody (10H11.E12) specifically recognizes phosphorylated Atm, making p-Atm (10H11.E12) monoclonal antibody an invaluable tool for studying DNA damage response mechanisms and pathophysiology of related disorders.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

p-Atm Antibody (10H11.E12) References:

1. ATM signaling and genomic stability in response to DNA damage.  |  Lavin, MF., et al. 2005. Mutat Res. 569: 123-32. PMID: 15603757
2. Identification and characterization of a novel and specific inhibitor of the ataxia-telangiectasia mutated kinase ATM.  |  Hickson, I., et al. 2004. Cancer Res. 64: 9152-9. PMID: 15604286
3. Alterations of DNA damage-response genes ATM and ATR in pyothorax-associated lymphoma.  |  Liu, A., et al. 2005. Lab Invest. 85: 436-46. PMID: 15696190
4. Ataxia-telangiectasia-mutated dependent phosphorylation of Artemis in response to DNA damage.  |  Chen, L., et al. 2005. Cancer Sci. 96: 134-41. PMID: 15723659
5. ATM activation in normal human tissues and testicular cancer.  |  Bartkova, J., et al. 2005. Cell Cycle. 4: 838-45. PMID: 15846060
6. Reduced expression of phosphorylated ataxia-telangiectasia mutated gene is related to poor prognosis and gemcitabine chemoresistance in pancreatic cancer.  |  Xun, J., et al. 2023. BMC Cancer. 23: 835. PMID: 37674118
7. PIK-related kinases: DNA repair, recombination, and cell cycle checkpoints.  |  Keith, CT. and Schreiber, SL. 1995. Science. 270: 50-1. PMID: 7569949
8. DNA-dependent protein kinase catalytic subunit: a relative of phosphatidylinositol 3-kinase and the ataxia telangiectasia gene product.  |  Hartley, KO., et al. 1995. Cell. 82: 849-56. PMID: 7671312
9. Discovery of AT gene sparks biomedical research bonanza.  |  Nowak, R. 1995. Science. 268: 1700-1. PMID: 7792589
10. A single ataxia telangiectasia gene with a product similar to PI-3 kinase.  |  Savitsky, K., et al. 1995. Science. 268: 1749-53. PMID: 7792600

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-p-Atm Antibody (10H11.E12) has 126 citations in a variety of scientific publications.

Primary antibodies like Anti-p-Atm Antibody (10H11.E12) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

p-Atm Antibody (10H11.E12) Specifications

Alternate Product Name: ATM

Product Clone Name: 10H11.E12

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: Ser 1981

Epitope Target: Atm

Epitope Species: human

Product Reactivity/Detection: Ser 1981 phosphorylated Atm

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P)

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 126 citations in various scientific publications

Available Conjugates/Forms for p-Atm Antibody (10H11.E12): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin