OTUD7B Antibody (H-4): sc-514402
- OTUD7B Antibody (H-4) is a mouse monoclonal IgG1 κ OTUD7B antibody, cited in 3 publications, provided at 200 µg/ml
- specific for an epitope mapping between amino acids 769-788 near the C-terminus of OTUD7B of human origin
- OTUD7B Antibody (H-4) is recommended for detection of OTUD7B of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-OTUD7B Antibody (H-4) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for OTUD7B Antibody (H-4) for WB applications. These reagents are now offered in bundles with OTUD7B Antibody (H-4) (see ordering information below).
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OTUD7B Antibody (H-4) is a mouse monoclonal IgG1 kappa light chain antibody that detects OTUD7B protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). OTUD7B monoclonal antibody (H-4) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. OTUD7B protein, also known as OTU domain-containing protein 7B, ZA20D1, or Cezanne, is an 843 amino acid protein primarily localized in the nucleus and cytoplasm, playing a crucial role in cellular processes. OTUD7B′s ability to hydrolyze branched and linear forms of polyubiquitin, particularly deubiquitinating Lys-48- and Lys-63-linked polyubiquitin chains, is vital for regulating inflammatory response within cells. By modulating ubiquitination status of target proteins, OTUD7B helps maintain cellular homeostasis and influences cell survival, particularly in inflammation contexts. OTUD7B is involved in a negative feedback loop in pro-inflammatory signaling, which suppresses NFκB activity and aids in resolving inflammatory responses. OTUD7B′s structure includes one C-terminal A20-type zinc finger, one OTU domain, and one N-terminal TRAF-binding domain, all essential for deubiquitinating activity and functional interactions with other signaling pathways.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
OTUD7B Antibody (H-4) References:
- Isolation and characterization of two novel A20-like proteins. | Evans, PC., et al. 2001. Biochem J. 357: 617-23. PMID: 11463333
- A novel type of deubiquitinating enzyme. | Evans, PC., et al. 2003. J Biol Chem. 278: 23180-6. PMID: 12682062
- Zinc-finger protein A20, a regulator of inflammation and cell survival, has de-ubiquitinating activity. | Evans, PC., et al. 2004. Biochem J. 378: 727-34. PMID: 14748687
- A common 93-kb duplicated DNA sequence at 1q21.2 in acute lymphoblastic leukemia and Burkitt lymphoma. | La Starza, R., et al. 2007. Cancer Genet Cytogenet. 175: 73-6. PMID: 17498563
- NF-kappaB suppression by the deubiquitinating enzyme Cezanne: a novel negative feedback loop in pro-inflammatory signaling. | Enesa, K., et al. 2008. J Biol Chem. 283: 7036-45. PMID: 18178551
- Hydrogen peroxide prolongs nuclear localization of NF-kappaB in activated cells by suppressing negative regulatory mechanisms. | Enesa, K., et al. 2008. J Biol Chem. 283: 18582-90. PMID: 18474597
- The cytokine-inducible zinc finger protein A20 inhibits IL-1-induced NF-kappaB activation at the level of TRAF6. | Heyninck, K. and Beyaert, R. 1999. FEBS Lett. 442: 147-50. PMID: 9928991
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
OTUD7B Antibody (H-4) | sc-514402 | 200 µg/ml | $316.00 | |||
OTUD7B Antibody (H-4): m-IgG Fc BP-HRP Bundle | sc-531111 | 200 µg Ab; 10 µg BP | $354.00 | |||
OTUD7B Antibody (H-4): m-IgGκ BP-HRP Bundle | sc-524710 | 200 µg Ab, 40 µg BP | $354.00 | |||
OTUD7B Antibody (H-4) AC | sc-514402 AC | 500 µg/ml, 25% agarose | $416.00 | |||
OTUD7B Antibody (H-4) HRP | sc-514402 HRP | 200 µg/ml | $316.00 | |||
OTUD7B Antibody (H-4) FITC | sc-514402 FITC | 200 µg/ml | $330.00 | |||
OTUD7B Antibody (H-4) PE | sc-514402 PE | 200 µg/ml | $343.00 | |||
OTUD7B Antibody (H-4) Alexa Fluor® 488 | sc-514402 AF488 | 200 µg/ml | $357.00 | |||
OTUD7B Antibody (H-4) Alexa Fluor® 546 | sc-514402 AF546 | 200 µg/ml | $357.00 | |||
OTUD7B Antibody (H-4) Alexa Fluor® 594 | sc-514402 AF594 | 200 µg/ml | $357.00 | |||
OTUD7B Antibody (H-4) Alexa Fluor® 647 | sc-514402 AF647 | 200 µg/ml | $357.00 | |||
OTUD7B Antibody (H-4) Alexa Fluor® 680 | sc-514402 AF680 | 200 µg/ml | $357.00 | |||
OTUD7B Antibody (H-4) Alexa Fluor® 790 | sc-514402 AF790 | 200 µg/ml | $357.00 | |||
OTUD7B (H-4) Neutralizing Peptide | sc-514402 P | 100 µg/0.5 ml | $68.00 |