OTC Antibody (E-9): sc-515791

OTC Antibody (E-9) is a mouse monoclonal IgG2b kappa light chain antibody that detects OTC protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-OTC antibody (E-9) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. OTC, also known as ornithine carbamoyltransferase or OTCase, is a crucial 354 amino acid enzyme that belongs to the ATCase/OTCase family and is primarily expressed in the liver and intestinal mucosa, where OTC localizes to the mitochondrial matrix as a homotrimer. OTC plays an essential role in the urea cycle, catalyzing the conversion of L-ornithine and carbamoyl phosphate into L-citrulline, a critical step for the detoxification of ammonia in humans. Disruptions in the OTC gene can lead to ornithine carbamoyltransferase deficiency (OTCD), an X-linked disorder characterized by hyperammonemia, which can be fatal in males and presents with variable symptoms in females. The OTC gene is located near a region of the X chromosome associated with Duchenne muscular dystrophy, indicating a potential link between these two conditions. Anti-OTC antibody (E-9) in multiple formats serves as a versatile tool for researchers studying the urea cycle and related metabolic disorders.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

OTC Antibody (E-9) References:

1. Ornithine transcarbamylase deficiency unmasked because of gastrointestinal bleeding.  |  Trivedi, M., et al. 2001. J Clin Gastroenterol. 32: 340-3. PMID: 11276280
2. Six new mutations in the ornithine transcarbamylase gene detected by single-strand conformational polymorphism.  |  Tuchman, M., et al. 1992. Pediatr Res. 32: 600-4. PMID: 1480464
3. Mutations and polymorphisms in the human ornithine transcarbamylase (OTC) gene.  |  Yamaguchi, S., et al. 2006. Hum Mutat. 27: 626-32. PMID: 16786505
4. In vitro demonstration of intra-locus compensation using the ornithine transcarbamylase protein as model.  |  Suriano, G., et al. 2007. Hum Mol Genet. 16: 2209-14. PMID: 17613537
5. Postchemotherapy hyperammonemic encephalopathy emulating ornithine transcarbamoylase (OTC) deficiency.  |  Chan, JS., et al. 2008. South Med J. 101: 543-5. PMID: 18414167
6. Isolation and characterization of the human ornithine transcarbamylase gene: structure of the 5'-end region.  |  Hata, A., et al. 1986. J Biochem. 100: 717-25. PMID: 3782067
7. Human ornithine transcarbamylase locus mapped to band Xp21.1 near the Duchenne muscular dystrophy locus.  |  Lindgren, V., et al. 1984. Science. 226: 698-700. PMID: 6494904

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-OTC Antibody (E-9) has 2 citations in a variety of scientific publications.

Primary antibodies like Anti-OTC Antibody (E-9) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

OTC Antibody (E-9) Specifications

Alternate Product Name: OTC

Product Clone Name: E-9

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 55-354

Epitope Target: OTC

Epitope Species: human

Product Reactivity/Detection: OTC

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 2 citations in various scientific publications

Available Conjugates/Forms for OTC Antibody (E-9): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin